Skip to content

Question about REDItools v1 and v3 #28

@andyjslee

Description

@andyjslee

I was wondering how you process the GENCODE GTF file (e.g. v41 human) for use with REDItools AnnotateTable.py. It seems that newer GTF versions omit the transcript_id field, which REDItools (being an older tool) appears to depend on.

When I filter out lines missing transcript_id in the GENCODE GTF file, AnnotateTable.py runs but I'm not sure if the outputs are correct.

In a simulated dataset, I have found that v1 is more sensitive and so I'd like to use v1. REDItools v1 also sets the strand information correctly whereas v3 infers * for the strand. Are there major differences between v1 and v3 in the underlying algorithms?

Thank you for your help!

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions