ZeroDivisionError in st_pipeline

[jocelynnijin]

Hi! Thank you so much for building this tool!

I am trying to run the following:
st_pipeline_run
--output-folder $OUTPUT_DIR
--ids $WHITE_LIST
--ref-map $GENOME_DIR
--ref-annotation $GTF_FILE
--expName $SAMPLE
--verbose
--log-file $OUTPUT_DIR/${SAMPLE}_log.txt
--demultiplexing-kmer 5
--temp-folder $TMP_ST
--no-clean-up
--umi-start-position 16
--umi-end-position 26
--demultiplexing-overhang 0
--min-length-qual-trimming 18 \

However, I kept encountering the following error message:

"ST Pipeline, parameters loaded
ST Pipeline, logger created
ST Pipeline, sanity check passed. Starting the run...
Error running the pipeline
Processing records: 0record [00:00, ?record/s]
Traceback (most recent call last):
File "/home/nij2/miniconda3/envs/stpipeline/bin/taggd_demultiplex", line 8, in
sys.exit(main())
File "/home/nij2/miniconda3/envs/stpipeline/lib/python3.10/site-packages/scripts/taggd_demultiplex.py", line 447, in main
print(str(demux.stats))
File "/home/nij2/miniconda3/envs/stpipeline/lib/python3.10/site-packages/taggd/core/statistics.py", line 40, in str
f"# Matches: {mat} [{mat * 100.0 / self.total_reads:.2f}%]\n"
ZeroDivisionError: float division by zero
"

My input files should be right. Please let me know if there’s anything I might be overlooking, thank you in advance for the help and support!!

Sincerely,
Jocelyn