diff --git a/dev-packages/e2e-tests/test-applications/tanstackstart-react/src/routes/api.hello.ts b/dev-packages/e2e-tests/test-applications/tanstackstart-react/src/routes/api.hello.ts
new file mode 100644
index 000000000000..6508439a9b33
--- /dev/null
+++ b/dev-packages/e2e-tests/test-applications/tanstackstart-react/src/routes/api.hello.ts
@@ -0,0 +1,11 @@
+import { createFileRoute } from '@tanstack/react-router';
+
+export const Route = createFileRoute('/api/hello')({
+  server: {
+    handlers: {
+      GET: async () => {
+        return new Response('Hello, world!');
+      },
+    },
+  },
+});
diff --git a/dev-packages/e2e-tests/test-applications/tanstackstart-react/tests/transaction.test.ts b/dev-packages/e2e-tests/test-applications/tanstackstart-react/tests/transaction.test.ts
index d2ebbffb0ec0..78e860e309b3 100644
--- a/dev-packages/e2e-tests/test-applications/tanstackstart-react/tests/transaction.test.ts
+++ b/dev-packages/e2e-tests/test-applications/tanstackstart-react/tests/transaction.test.ts
@@ -98,3 +98,37 @@ test('Sends a server function transaction for a nested server function only if i
   expect(nestedSpan).toBeDefined();
   expect(nestedSpan?.parent_span_id).toBe(autoSpan?.span_id);
 });
+
+test('Sends an API route transaction with auto-instrumentation', async ({ page }) => {
+  const transactionEventPromise = waitForTransaction('tanstackstart-react', transactionEvent => {
+    return (
+      transactionEvent?.contexts?.trace?.op === 'http.server' && transactionEvent?.transaction === 'GET /api/hello'
+    );
+  });
+
+  await page.goto('/api/hello');
+
+  const transactionEvent = await transactionEventPromise;
+
+  expect(transactionEvent).toEqual(
+    expect.objectContaining({
+      transaction: 'GET /api/hello',
+    }),
+  );
+
+  expect(transactionEvent?.spans).toEqual(
+    expect.arrayContaining([
+      expect.objectContaining({
+        description: 'GET /api/hello',
+        op: 'http.server',
+        origin: 'auto.http.tanstackstart.server',
+        data: {
+          'sentry.op': 'http.server',
+          'sentry.origin': 'auto.http.tanstackstart.server',
+          'sentry.source': 'url',
+          'http.request.method': 'GET',
+        },
+      }),
+    ]),
+  );
+});
diff --git a/packages/tanstackstart-react/src/server/wrapFetchWithSentry.ts b/packages/tanstackstart-react/src/server/wrapFetchWithSentry.ts
index 22d218ef0b48..788873db9970 100644
--- a/packages/tanstackstart-react/src/server/wrapFetchWithSentry.ts
+++ b/packages/tanstackstart-react/src/server/wrapFetchWithSentry.ts
@@ -1,4 +1,11 @@
-import { SEMANTIC_ATTRIBUTE_SENTRY_OP, SEMANTIC_ATTRIBUTE_SENTRY_ORIGIN, startSpan } from '@sentry/node';
+import type { SpanAttributes } from '@sentry/core';
+import { SEMANTIC_ATTRIBUTE_HTTP_REQUEST_METHOD } from '@sentry/core';
+import {
+  SEMANTIC_ATTRIBUTE_SENTRY_OP,
+  SEMANTIC_ATTRIBUTE_SENTRY_ORIGIN,
+  SEMANTIC_ATTRIBUTE_SENTRY_SOURCE,
+  startSpan,
+} from '@sentry/node';
 import { extractServerFunctionSha256 } from './utils';
 
 export type ServerEntry = {
@@ -37,30 +44,39 @@ export function wrapFetchWithSentry(serverEntry: ServerEntry): ServerEntry {
         const url = new URL(request.url);
         const method = request.method || 'GET';
 
-        // instrument server functions
+        let op: string;
+        let spanAttributes: SpanAttributes;
+
         if (url.pathname.includes('_serverFn') || url.pathname.includes('createServerFn')) {
+          // server function call
+          op = 'function.tanstackstart';
           const functionSha256 = extractServerFunctionSha256(url.pathname);
-          const op = 'function.tanstackstart';
-
-          const serverFunctionSpanAttributes = {
+          spanAttributes = {
             [SEMANTIC_ATTRIBUTE_SENTRY_ORIGIN]: 'auto.function.tanstackstart.server',
             [SEMANTIC_ATTRIBUTE_SENTRY_OP]: op,
             'tanstackstart.function.hash.sha256': functionSha256,
           };
-
-          return startSpan(
-            {
-              op: op,
-              name: `${method} ${url.pathname}`,
-              attributes: serverFunctionSpanAttributes,
-            },
-            () => {
-              return target.apply(thisArg, args);
-            },
-          );
+        } else {
+          // API route or other server request
+          op = 'http.server';
+          spanAttributes = {
+            [SEMANTIC_ATTRIBUTE_SENTRY_ORIGIN]: 'auto.http.tanstackstart.server',
+            [SEMANTIC_ATTRIBUTE_SENTRY_OP]: op,
+            [SEMANTIC_ATTRIBUTE_HTTP_REQUEST_METHOD]: method,
+            [SEMANTIC_ATTRIBUTE_SENTRY_SOURCE]: 'url',
+          };
         }
 
-        return target.apply(thisArg, args);
+        return startSpan(
+          {
+            op,
+            name: `${method} ${url.pathname}`,
+            attributes: spanAttributes,
+          },
+          () => {
+            return target.apply(thisArg, args);
+          },
+        );
       },
     });
   }