From 0cbf5a36fa0d98051e4217aa7356ec1486a0e083 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Thu, 20 Nov 2025 09:32:12 +0000
Subject: [PATCH 1/4] Template update for nf-core/tools version 3.5.1

---
 .github/workflows/awsfulltest.yml             |  2 +-
 .github/workflows/awstest.yml                 |  2 +-
 .github/workflows/download_pipeline.yml       |  2 +-
 .github/workflows/fix_linting.yml             |  2 +-
 .github/workflows/linting.yml                 |  6 +--
 .github/workflows/nf-test.yml                 |  4 +-
 .github/workflows/release-announcements.yml   |  9 ++---
 .../workflows/template-version-comment.yml    |  2 +-
 .nf-core.yml                                  |  2 +-
 .prettierignore                               |  2 +
 README.md                                     |  4 +-
 modules.json                                  |  4 +-
 modules/nf-core/multiqc/environment.yml       |  2 +-
 modules/nf-core/multiqc/main.nf               |  4 +-
 .../nf-core/multiqc/tests/main.nf.test.snap   | 24 ++++++------
 nextflow.config                               |  2 -
 ro-crate-metadata.json                        | 14 +++----
 .../utils_nfcore_deepmutscan_pipeline/main.nf |  6 +--
 .../nf-core/utils_nfcore_pipeline/main.nf     |  2 +-
 workflows/deepmutscan.nf                      | 38 ++++++++++++++-----
 20 files changed, 74 insertions(+), 59 deletions(-)

diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index e7c08d6..04028fa 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -40,7 +40,7 @@ jobs:
             }
           profiles: test_full
 
-      - uses: actions/upload-artifact@ea165f8d65b6e75b540449e92b4886f43607fa02 # v4
+      - uses: actions/upload-artifact@330a01c490aca151604b8cf639adc76d48f6c5d4 # v5
         with:
           name: Seqera Platform debug log file
           path: |
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index e82b4c0..9e687d3 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -25,7 +25,7 @@ jobs:
             }
           profiles: test
 
-      - uses: actions/upload-artifact@ea165f8d65b6e75b540449e92b4886f43607fa02 # v4
+      - uses: actions/upload-artifact@330a01c490aca151604b8cf639adc76d48f6c5d4 # v5
         with:
           name: Seqera Platform debug log file
           path: |
diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml
index 6d94bcb..45884ff 100644
--- a/.github/workflows/download_pipeline.yml
+++ b/.github/workflows/download_pipeline.yml
@@ -127,7 +127,7 @@ jobs:
           fi
 
       - name: Upload Nextflow logfile for debugging purposes
-        uses: actions/upload-artifact@ea165f8d65b6e75b540449e92b4886f43607fa02 # v4
+        uses: actions/upload-artifact@330a01c490aca151604b8cf639adc76d48f6c5d4 # v5
         with:
           name: nextflow_logfile.txt
           path: .nextflow.log*
diff --git a/.github/workflows/fix_linting.yml b/.github/workflows/fix_linting.yml
index 65b1dc7..ea03315 100644
--- a/.github/workflows/fix_linting.yml
+++ b/.github/workflows/fix_linting.yml
@@ -13,7 +13,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       # Use the @nf-core-bot token to check out so we can push later
-      - uses: actions/checkout@08c6903cd8c0fde910a37f88322edcfb5dd907a8 # v5
+      - uses: actions/checkout@93cb6efe18208431cddfb8368fd83d5badbf9bfd # v5
         with:
           token: ${{ secrets.nf_core_bot_auth_token }}
 
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 30e6602..7a527a3 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -11,7 +11,7 @@ jobs:
   pre-commit:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@08c6903cd8c0fde910a37f88322edcfb5dd907a8 # v5
+      - uses: actions/checkout@93cb6efe18208431cddfb8368fd83d5badbf9bfd # v5
 
       - name: Set up Python 3.14
         uses: actions/setup-python@e797f83bcb11b83ae66e0230d6156d7c80228e7c # v6
@@ -28,7 +28,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@08c6903cd8c0fde910a37f88322edcfb5dd907a8 # v5
+        uses: actions/checkout@93cb6efe18208431cddfb8368fd83d5badbf9bfd # v5
 
       - name: Install Nextflow
         uses: nf-core/setup-nextflow@v2
@@ -71,7 +71,7 @@ jobs:
 
       - name: Upload linting log file artifact
         if: ${{ always() }}
-        uses: actions/upload-artifact@ea165f8d65b6e75b540449e92b4886f43607fa02 # v4
+        uses: actions/upload-artifact@330a01c490aca151604b8cf639adc76d48f6c5d4 # v5
         with:
           name: linting-logs
           path: |
diff --git a/.github/workflows/nf-test.yml b/.github/workflows/nf-test.yml
index e20bf6d..c98d76e 100644
--- a/.github/workflows/nf-test.yml
+++ b/.github/workflows/nf-test.yml
@@ -40,7 +40,7 @@ jobs:
           rm -rf ./* || true
           rm -rf ./.??* || true
           ls -la ./
-      - uses: actions/checkout@08c6903cd8c0fde910a37f88322edcfb5dd907a8 # v5
+      - uses: actions/checkout@93cb6efe18208431cddfb8368fd83d5badbf9bfd # v5
         with:
           fetch-depth: 0
 
@@ -85,7 +85,7 @@ jobs:
       TOTAL_SHARDS: ${{ needs.nf-test-changes.outputs.total_shards }}
 
     steps:
-      - uses: actions/checkout@08c6903cd8c0fde910a37f88322edcfb5dd907a8 # v5
+      - uses: actions/checkout@93cb6efe18208431cddfb8368fd83d5badbf9bfd # v5
         with:
           fetch-depth: 0
 
diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml
index e64cebd..431d3d4 100644
--- a/.github/workflows/release-announcements.yml
+++ b/.github/workflows/release-announcements.yml
@@ -15,10 +15,9 @@ jobs:
           echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')" | sed 's/-//g' >> $GITHUB_OUTPUT
 
       - name: get description
-        id: get_topics
+        id: get_description
         run: |
-          echo "description=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .description' >> $GITHUB_OUTPUT
-
+          echo "description=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .description')" >> $GITHUB_OUTPUT
       - uses: rzr/fediverse-action@master
         with:
           access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }}
@@ -27,9 +26,7 @@ jobs:
           # https://docs.github.com/en/developers/webhooks-and-events/webhooks/webhook-events-and-payloads#release
           message: |
             Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}!
-
-            ${{ steps.get_topics.outputs.description }}
-
+            ${{ steps.get_description.outputs.description }}
             Please see the changelog: ${{ github.event.release.html_url }}
 
             ${{ steps.get_topics.outputs.topics }} #nfcore #openscience #nextflow #bioinformatics
diff --git a/.github/workflows/template-version-comment.yml b/.github/workflows/template-version-comment.yml
index c5988af..e8560fc 100644
--- a/.github/workflows/template-version-comment.yml
+++ b/.github/workflows/template-version-comment.yml
@@ -9,7 +9,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@08c6903cd8c0fde910a37f88322edcfb5dd907a8 # v5
+        uses: actions/checkout@93cb6efe18208431cddfb8368fd83d5badbf9bfd # v5
         with:
           ref: ${{ github.event.pull_request.head.sha }}
 
diff --git a/.nf-core.yml b/.nf-core.yml
index 3251f8f..a24b80a 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -1,5 +1,5 @@
 lint: {}
-nf_core_version: 3.4.1
+nf_core_version: 3.5.1
 repository_type: pipeline
 template:
   author: Benjamin Wehnert & Max Stammnitz
diff --git a/.prettierignore b/.prettierignore
index 2255e3e..dd749d4 100644
--- a/.prettierignore
+++ b/.prettierignore
@@ -12,3 +12,5 @@ testing*
 bin/
 .nf-test/
 ro-crate-metadata.json
+modules/nf-core/
+subworkflows/nf-core/
diff --git a/README.md b/README.md
index a5b2e75..4e8dc52 100644
--- a/README.md
+++ b/README.md
@@ -5,13 +5,13 @@
   </picture>
 </h1>
 
-[![Open in GitHub Codespaces](https://github.com/codespaces/badge.svg)](https://github.com/codespaces/new/nf-core/deepmutscan)
+[![Open in GitHub Codespaces](https://img.shields.io/badge/Open_In_GitHub_Codespaces-black?labelColor=grey&logo=github)](https://github.com/codespaces/new/nf-core/deepmutscan)
 [![GitHub Actions CI Status](https://github.com/nf-core/deepmutscan/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/deepmutscan/actions/workflows/nf-test.yml)
 [![GitHub Actions Linting Status](https://github.com/nf-core/deepmutscan/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/deepmutscan/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/deepmutscan/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
 [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)
 
 [![Nextflow](https://img.shields.io/badge/version-%E2%89%A525.04.0-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)
-[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.4.1-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.4.1)
+[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.5.1-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.5.1)
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
 [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
 [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
diff --git a/modules.json b/modules.json
index 7c159e0..efc56c9 100644
--- a/modules.json
+++ b/modules.json
@@ -12,7 +12,7 @@
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "e10b76ca0c66213581bec2833e30d31f239dec0b",
+                        "git_sha": "af27af1be706e6a2bb8fe454175b0cdf77f47b49",
                         "installed_by": ["modules"]
                     }
                 }
@@ -26,7 +26,7 @@
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "271e7fc14eb1320364416d996fb077421f3faed2",
                         "installed_by": ["subworkflows"]
                     },
                     "utils_nfschema_plugin": {
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
index dd513cb..d02016a 100644
--- a/modules/nf-core/multiqc/environment.yml
+++ b/modules/nf-core/multiqc/environment.yml
@@ -4,4 +4,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::multiqc=1.31
+  - bioconda::multiqc=1.32
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 5288f5c..c1158fb 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -3,8 +3,8 @@ process MULTIQC {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ef/eff0eafe78d5f3b65a6639265a16b89fdca88d06d18894f90fcdb50142004329/data' :
-        'community.wave.seqera.io/library/multiqc:1.31--1efbafd542a23882' }"
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/8c/8c6c120d559d7ee04c7442b61ad7cf5a9e8970be5feefb37d68eeaa60c1034eb/data' :
+        'community.wave.seqera.io/library/multiqc:1.32--d58f60e4deb769bf' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
index 17881d1..a88bafd 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test.snap
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -2,14 +2,14 @@
     "multiqc_versions_single": {
         "content": [
             [
-                "versions.yml:md5,8968b114a3e20756d8af2b80713bcc4f"
+                "versions.yml:md5,737bb2c7cad54ffc2ec020791dc48b8f"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.3",
+            "nextflow": "24.10.4"
         },
-        "timestamp": "2025-09-08T20:57:36.139055243"
+        "timestamp": "2025-10-27T13:33:24.356715"
     },
     "multiqc_stub": {
         "content": [
@@ -17,25 +17,25 @@
                 "multiqc_report.html",
                 "multiqc_data",
                 "multiqc_plots",
-                "versions.yml:md5,8968b114a3e20756d8af2b80713bcc4f"
+                "versions.yml:md5,737bb2c7cad54ffc2ec020791dc48b8f"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.3",
+            "nextflow": "24.10.4"
         },
-        "timestamp": "2025-09-08T20:59:15.142230631"
+        "timestamp": "2025-10-27T13:34:11.103619"
     },
     "multiqc_versions_config": {
         "content": [
             [
-                "versions.yml:md5,8968b114a3e20756d8af2b80713bcc4f"
+                "versions.yml:md5,737bb2c7cad54ffc2ec020791dc48b8f"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.3",
+            "nextflow": "24.10.4"
         },
-        "timestamp": "2025-09-08T20:58:29.629087066"
+        "timestamp": "2025-10-27T13:34:04.615233"
     }
 }
\ No newline at end of file
diff --git a/nextflow.config b/nextflow.config
index e242f5a..0975f17 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -170,8 +170,6 @@ profiles {
     test_full { includeConfig 'conf/test_full.config' }
 }
 
-// Set AWS client to anonymous when using the default igenomes_base
-aws.client.anonymous = !params.igenomes_ignore && params.igenomes_base?.startsWith('s3://ngi-igenomes/igenomes/') ?: false
 // Load nf-core custom profiles from different institutions
 
 // If params.custom_config_base is set AND either the NXF_OFFLINE environment variable is not set or params.custom_config_base is a local path, the nfcore_custom.config file from the specified base path is included.
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index 768ef3a..57aaf4e 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -22,8 +22,8 @@
             "@id": "./",
             "@type": "Dataset",
             "creativeWorkStatus": "Stable",
-            "datePublished": "2025-11-11T21:50:24+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-deepmutscan_logo_dark.png\">\n    <img alt=\"nf-core/deepmutscan\" src=\"docs/images/nf-core-deepmutscan_logo_light.png\">\n  </picture>\n</h1>\n\n[![Open in GitHub Codespaces](https://github.com/codespaces/badge.svg)](https://github.com/codespaces/new/nf-core/deepmutscan)\n[![GitHub Actions CI Status](https://github.com/nf-core/deepmutscan/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/deepmutscan/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/deepmutscan/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/deepmutscan/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/deepmutscan/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A525.04.0-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.4.1-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.4.1)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/deepmutscan)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23deepmutscan-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/deepmutscan)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/deepmutscan** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/guidelines/graphic_design/workflow_diagrams#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/deepmutscan \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/deepmutscan/usage) and the [parameter documentation](https://nf-co.re/deepmutscan/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/deepmutscan/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/deepmutscan/output).\n\n## Credits\n\nnf-core/deepmutscan was originally written by Benjamin Wehnert & Max Stammnitz.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#deepmutscan` channel](https://nfcore.slack.com/channels/deepmutscan) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/deepmutscan for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->\n\n<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
+            "datePublished": "2025-11-20T09:32:07+00:00",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-deepmutscan_logo_dark.png\">\n    <img alt=\"nf-core/deepmutscan\" src=\"docs/images/nf-core-deepmutscan_logo_light.png\">\n  </picture>\n</h1>\n\n[![Open in GitHub Codespaces](https://img.shields.io/badge/Open_In_GitHub_Codespaces-black?labelColor=grey&logo=github)](https://github.com/codespaces/new/nf-core/deepmutscan)\n[![GitHub Actions CI Status](https://github.com/nf-core/deepmutscan/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/deepmutscan/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/deepmutscan/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/deepmutscan/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/deepmutscan/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A525.04.0-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.5.1-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.5.1)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/deepmutscan)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23deepmutscan-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/deepmutscan)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/deepmutscan** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/guidelines/graphic_design/workflow_diagrams#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/deepmutscan \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/deepmutscan/usage) and the [parameter documentation](https://nf-co.re/deepmutscan/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/deepmutscan/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/deepmutscan/output).\n\n## Credits\n\nnf-core/deepmutscan was originally written by Benjamin Wehnert & Max Stammnitz.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#deepmutscan` channel](https://nfcore.slack.com/channels/deepmutscan) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/deepmutscan for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->\n\n<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
             "hasPart": [
                 {
                     "@id": "main.nf"
@@ -99,7 +99,7 @@
             },
             "mentions": [
                 {
-                    "@id": "#cacc4214-323e-45a9-ae87-e315532ca604"
+                    "@id": "#280e9a69-af96-4e4c-80bc-ae5ef9dda531"
                 }
             ],
             "name": "nf-core/deepmutscan"
@@ -123,7 +123,7 @@
             "@id": "main.nf",
             "@type": ["File", "SoftwareSourceCode", "ComputationalWorkflow"],
             "dateCreated": "",
-            "dateModified": "2025-11-11T21:50:24Z",
+            "dateModified": "2025-11-20T09:32:07Z",
             "dct:conformsTo": "https://bioschemas.org/profiles/ComputationalWorkflow/1.0-RELEASE/",
             "keywords": [
                 "nf-core",
@@ -157,11 +157,11 @@
             "version": "!>=25.04.0"
         },
         {
-            "@id": "#cacc4214-323e-45a9-ae87-e315532ca604",
+            "@id": "#280e9a69-af96-4e4c-80bc-ae5ef9dda531",
             "@type": "TestSuite",
             "instance": [
                 {
-                    "@id": "#c635b954-bdef-4988-90ff-319caec590b2"
+                    "@id": "#ac596659-2e2a-421a-b5d0-69df6db85229"
                 }
             ],
             "mainEntity": {
@@ -170,7 +170,7 @@
             "name": "Test suite for nf-core/deepmutscan"
         },
         {
-            "@id": "#c635b954-bdef-4988-90ff-319caec590b2",
+            "@id": "#ac596659-2e2a-421a-b5d0-69df6db85229",
             "@type": "TestInstance",
             "name": "GitHub Actions workflow for testing nf-core/deepmutscan",
             "resource": "repos/nf-core/deepmutscan/actions/workflows/nf-test.yml",
diff --git a/subworkflows/local/utils_nfcore_deepmutscan_pipeline/main.nf b/subworkflows/local/utils_nfcore_deepmutscan_pipeline/main.nf
index f0ae451..3b2c26a 100644
--- a/subworkflows/local/utils_nfcore_deepmutscan_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_deepmutscan_pipeline/main.nf
@@ -39,7 +39,7 @@ workflow PIPELINE_INITIALISATION {
 
     main:
 
-    ch_versions = Channel.empty()
+    ch_versions = channel.empty()
 
     //
     // Print version and exit if required and dump pipeline parameters to JSON file
@@ -64,7 +64,7 @@ workflow PIPELINE_INITIALISATION {
 \033[0;35m  nf-core/deepmutscan ${workflow.manifest.version}\033[0m
 -\033[2m----------------------------------------------------\033[0m-
 """
-    after_text = """${workflow.manifest.doi ? "\n* The pipeline\n" : ""}${workflow.manifest.doi.tokenize(",").collect { "    https://doi.org/${it.trim().replace('https://doi.org/','')}"}.join("\n")}${workflow.manifest.doi ? "\n" : ""}
+    after_text = """${workflow.manifest.doi ? "\n* The pipeline\n" : ""}${workflow.manifest.doi.tokenize(",").collect { doi -> "    https://doi.org/${doi.trim().replace('https://doi.org/','')}"}.join("\n")}${workflow.manifest.doi ? "\n" : ""}
 * The nf-core framework
     https://doi.org/10.1038/s41587-020-0439-x
 
@@ -101,7 +101,7 @@ workflow PIPELINE_INITIALISATION {
     // Create channel from input file provided through params.input
     //
 
-    Channel
+    channel
         .fromList(samplesheetToList(params.input, "${projectDir}/assets/schema_input.json"))
         .map {
             meta, fastq_1, fastq_2 ->
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
index bfd2587..2f30e9a 100644
--- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
@@ -98,7 +98,7 @@ def workflowVersionToYAML() {
 // Get channel of software versions used in pipeline in YAML format
 //
 def softwareVersionsToYAML(ch_versions) {
-    return ch_versions.unique().map { version -> processVersionsFromYAML(version) }.unique().mix(Channel.of(workflowVersionToYAML()))
+    return ch_versions.unique().map { version -> processVersionsFromYAML(version) }.unique().mix(channel.of(workflowVersionToYAML()))
 }
 
 //
diff --git a/workflows/deepmutscan.nf b/workflows/deepmutscan.nf
index 2b6bf8d..12ac5d0 100644
--- a/workflows/deepmutscan.nf
+++ b/workflows/deepmutscan.nf
@@ -22,8 +22,8 @@ workflow DEEPMUTSCAN {
     ch_samplesheet // channel: samplesheet read in from --input
     main:
 
-    ch_versions = Channel.empty()
-    ch_multiqc_files = Channel.empty()
+    ch_versions = channel.empty()
+    ch_multiqc_files = channel.empty()
     //
     // MODULE: Run FastQC
     //
@@ -36,7 +36,25 @@ workflow DEEPMUTSCAN {
     //
     // Collate and save software versions
     //
-    softwareVersionsToYAML(ch_versions)
+    def topic_versions = Channel.topic("versions")
+        .distinct()
+        .branch { entry ->
+            versions_file: entry instanceof Path
+            versions_tuple: true
+        }
+
+    def topic_versions_string = topic_versions.versions_tuple
+        .map { process, tool, version ->
+            [ process[process.lastIndexOf(':')+1..-1], "  ${tool}: ${version}" ]
+        }
+        .groupTuple(by:0)
+        .map { process, tool_versions ->
+            tool_versions.unique().sort()
+            "${process}:\n${tool_versions.join('\n')}"
+        }
+
+    softwareVersionsToYAML(ch_versions.mix(topic_versions.versions_file))
+        .mix(topic_versions_string)
         .collectFile(
             storeDir: "${params.outdir}/pipeline_info",
             name: 'nf_core_'  +  'deepmutscan_software_'  + 'mqc_'  + 'versions.yml',
@@ -48,24 +66,24 @@ workflow DEEPMUTSCAN {
     //
     // MODULE: MultiQC
     //
-    ch_multiqc_config        = Channel.fromPath(
+    ch_multiqc_config        = channel.fromPath(
         "$projectDir/assets/multiqc_config.yml", checkIfExists: true)
     ch_multiqc_custom_config = params.multiqc_config ?
-        Channel.fromPath(params.multiqc_config, checkIfExists: true) :
-        Channel.empty()
+        channel.fromPath(params.multiqc_config, checkIfExists: true) :
+        channel.empty()
     ch_multiqc_logo          = params.multiqc_logo ?
-        Channel.fromPath(params.multiqc_logo, checkIfExists: true) :
-        Channel.empty()
+        channel.fromPath(params.multiqc_logo, checkIfExists: true) :
+        channel.empty()
 
     summary_params      = paramsSummaryMap(
         workflow, parameters_schema: "nextflow_schema.json")
-    ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params))
+    ch_workflow_summary = channel.value(paramsSummaryMultiqc(summary_params))
     ch_multiqc_files = ch_multiqc_files.mix(
         ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
     ch_multiqc_custom_methods_description = params.multiqc_methods_description ?
         file(params.multiqc_methods_description, checkIfExists: true) :
         file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
-    ch_methods_description                = Channel.value(
+    ch_methods_description                = channel.value(
         methodsDescriptionText(ch_multiqc_custom_methods_description))
 
     ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions)

From a6aa7fac9c7f825705951c2376827e973a7fc0f3 Mon Sep 17 00:00:00 2001
From: BenjaminWehnert1008 <benjamin.wehnert@stud.uni-heidelberg.de>
Date: Sat, 7 Feb 2026 17:49:55 +0100
Subject: [PATCH 2/4] Template update for nf-core/tools version 3.1.2

---
 .devcontainer/devcontainer.json               |  28 ++--
 .devcontainer/setup.sh                        |  13 --
 .editorconfig                                 |  37 +++++
 .github/CONTRIBUTING.md                       |   2 +-
 .github/actions/get-shards/action.yml         |  69 ---------
 .github/actions/nf-test/action.yml            | 111 --------------
 .github/workflows/awsfulltest.yml             |  51 +++++--
 .github/workflows/awstest.yml                 |  14 +-
 .github/workflows/ci.yml                      |  87 +++++++++++
 .github/workflows/clean-up.yml                |   2 +-
 .github/workflows/download_pipeline.yml       |  22 +--
 .../{fix_linting.yml => fix-linting.yml}      |  16 +-
 .github/workflows/linting.yml                 |  21 +--
 .github/workflows/linting_comment.yml         |   4 +-
 .github/workflows/nf-test.yml                 | 144 ------------------
 .github/workflows/release-announcements.yml   |  41 ++++-
 ...mment.yml => template_version_comment.yml} |   4 +-
 .gitpod.yml                                   |  10 ++
 .nf-core.yml                                  |   2 +-
 .pre-commit-config.yaml                       |  26 +---
 .prettierignore                               |   3 -
 .prettierrc.yml                               |   5 -
 README.md                                     |  10 +-
 assets/nf-core-deepmutscan_logo_light.png     | Bin 106741 -> 106639 bytes
 assets/schema_input.json                      |   4 +-
 conf/base.config                              |   6 +-
 docs/images/nf-core-deepmutscan_logo_dark.png | Bin 28308 -> 28299 bytes
 .../images/nf-core-deepmutscan_logo_light.png | Bin 23926 -> 23882 bytes
 docs/output.md                                |  11 +-
 docs/usage.md                                 |   2 +-
 main.nf                                       |   5 +-
 modules.json                                  |  10 +-
 modules/nf-core/fastqc/environment.yml        |   2 -
 modules/nf-core/fastqc/main.nf                |  20 +--
 modules/nf-core/fastqc/meta.yml               |  23 ++-
 modules/nf-core/fastqc/tests/tags.yml         |   2 +
 modules/nf-core/multiqc/environment.yml       |   4 +-
 modules/nf-core/multiqc/main.nf               |   4 +-
 modules/nf-core/multiqc/meta.yml              | 110 ++++++-------
 .../nf-core/multiqc/tests/main.nf.test.snap   |  24 +--
 modules/nf-core/multiqc/tests/tags.yml        |   2 +
 nextflow.config                               |  92 ++++++-----
 nextflow_schema.json                          |  12 --
 nf-test.config                                |  24 ---
 ro-crate-metadata.json                        |  43 ++++--
 .../utils_nfcore_deepmutscan_pipeline/main.nf |  36 +----
 .../utils_nextflow_pipeline/tests/tags.yml    |   2 +
 .../nf-core/utils_nfcore_pipeline/main.nf     |   2 +-
 .../utils_nfcore_pipeline/tests/tags.yml      |   2 +
 .../nf-core/utils_nfschema_plugin/main.nf     |  40 +----
 .../utils_nfschema_plugin/tests/main.nf.test  |  56 -------
 .../tests/nextflow.config                     |   4 +-
 tests/.nftignore                              |  12 --
 tests/default.nf.test                         |  33 ----
 tests/nextflow.config                         |  14 --
 workflows/deepmutscan.nf                      |  38 ++---
 56 files changed, 483 insertions(+), 878 deletions(-)
 delete mode 100755 .devcontainer/setup.sh
 create mode 100644 .editorconfig
 delete mode 100644 .github/actions/get-shards/action.yml
 delete mode 100644 .github/actions/nf-test/action.yml
 create mode 100644 .github/workflows/ci.yml
 rename .github/workflows/{fix_linting.yml => fix-linting.yml} (80%)
 delete mode 100644 .github/workflows/nf-test.yml
 rename .github/workflows/{template-version-comment.yml => template_version_comment.yml} (91%)
 create mode 100644 .gitpod.yml
 create mode 100644 modules/nf-core/fastqc/tests/tags.yml
 create mode 100644 modules/nf-core/multiqc/tests/tags.yml
 delete mode 100644 nf-test.config
 create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml
 delete mode 100644 tests/.nftignore
 delete mode 100644 tests/default.nf.test
 delete mode 100644 tests/nextflow.config

diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json
index 97c8c97..b290e09 100644
--- a/.devcontainer/devcontainer.json
+++ b/.devcontainer/devcontainer.json
@@ -1,20 +1,20 @@
 {
     "name": "nfcore",
-    "image": "nfcore/devcontainer:latest",
+    "image": "nfcore/gitpod:latest",
+    "remoteUser": "gitpod",
+    "runArgs": ["--privileged"],
 
-    "remoteUser": "root",
-    "privileged": true,
+    // Configure tool-specific properties.
+    "customizations": {
+        // Configure properties specific to VS Code.
+        "vscode": {
+            // Set *default* container specific settings.json values on container create.
+            "settings": {
+                "python.defaultInterpreterPath": "/opt/conda/bin/python"
+            },
 
-    "remoteEnv": {
-        // Workspace path on the host for mounting with docker-outside-of-docker
-        "LOCAL_WORKSPACE_FOLDER": "${localWorkspaceFolder}"
-    },
-
-    "onCreateCommand": "./.devcontainer/setup.sh",
-
-    "hostRequirements": {
-        "cpus": 4,
-        "memory": "16gb",
-        "storage": "32gb"
+            // Add the IDs of extensions you want installed when the container is created.
+            "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"]
+        }
     }
 }
diff --git a/.devcontainer/setup.sh b/.devcontainer/setup.sh
deleted file mode 100755
index 2ca6343..0000000
--- a/.devcontainer/setup.sh
+++ /dev/null
@@ -1,13 +0,0 @@
-#!/usr/bin/env bash
-
-# Customise the terminal command prompt
-echo "export PROMPT_DIRTRIM=2" >> $HOME/.bashrc
-echo "export PS1='\[\e[3;36m\]\w ->\[\e[0m\\] '" >> $HOME/.bashrc
-export PROMPT_DIRTRIM=2
-export PS1='\[\e[3;36m\]\w ->\[\e[0m\\] '
-
-# Update Nextflow
-nextflow self-update
-
-# Update welcome message
-echo "Welcome to the nf-core/deepmutscan devcontainer!" > /usr/local/etc/vscode-dev-containers/first-run-notice.txt
diff --git a/.editorconfig b/.editorconfig
new file mode 100644
index 0000000..6d9b74c
--- /dev/null
+++ b/.editorconfig
@@ -0,0 +1,37 @@
+root = true
+
+[*]
+charset = utf-8
+end_of_line = lf
+insert_final_newline = true
+trim_trailing_whitespace = true
+indent_size = 4
+indent_style = space
+
+[*.{md,yml,yaml,html,css,scss,js}]
+indent_size = 2
+
+# These files are edited and tested upstream in nf-core/modules
+[/modules/nf-core/**]
+charset = unset
+end_of_line = unset
+insert_final_newline = unset
+trim_trailing_whitespace = unset
+indent_style = unset
+[/subworkflows/nf-core/**]
+charset = unset
+end_of_line = unset
+insert_final_newline = unset
+trim_trailing_whitespace = unset
+indent_style = unset
+
+[/assets/email*]
+indent_size = unset
+
+# ignore python and markdown
+[*.{py,md}]
+indent_style = unset
+
+# ignore ro-crate metadata files
+[**/ro-crate-metadata.json]
+insert_final_newline = unset
diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
index dc13efb..12dd4a0 100644
--- a/.github/CONTRIBUTING.md
+++ b/.github/CONTRIBUTING.md
@@ -78,7 +78,7 @@ If you wish to contribute a new step, please use the following coding standards:
 5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core pipelines schema build` tool).
 6. Add sanity checks and validation for all relevant parameters.
 7. Perform local tests to validate that the new code works as expected.
-8. If applicable, add a new test in the `tests` directory.
+8. If applicable, add a new test command in `.github/workflow/ci.yml`.
 9. Update MultiQC config `assets/multiqc_config.yml` so relevant suffixes, file name clean up and module plots are in the appropriate order. If applicable, add a [MultiQC](https://https://multiqc.info/) module.
 10. Add a description of the output files and if relevant any appropriate images from the MultiQC report to `docs/output.md`.
 
diff --git a/.github/actions/get-shards/action.yml b/.github/actions/get-shards/action.yml
deleted file mode 100644
index 3408527..0000000
--- a/.github/actions/get-shards/action.yml
+++ /dev/null
@@ -1,69 +0,0 @@
-name: "Get number of shards"
-description: "Get the number of nf-test shards for the current CI job"
-inputs:
-  max_shards:
-    description: "Maximum number of shards allowed"
-    required: true
-  paths:
-    description: "Component paths to test"
-    required: false
-  tags:
-    description: "Tags to pass as argument for nf-test --tag parameter"
-    required: false
-outputs:
-  shard:
-    description: "Array of shard numbers"
-    value: ${{ steps.shards.outputs.shard }}
-  total_shards:
-    description: "Total number of shards"
-    value: ${{ steps.shards.outputs.total_shards }}
-runs:
-  using: "composite"
-  steps:
-    - name: Install nf-test
-      uses: nf-core/setup-nf-test@v1
-      with:
-        version: ${{ env.NFT_VER }}
-    - name: Get number of shards
-      id: shards
-      shell: bash
-      run: |
-        # Run nf-test with dynamic parameter
-        nftest_output=$(nf-test test \
-          --profile +docker \
-          $(if [ -n "${{ inputs.tags }}" ]; then echo "--tag ${{ inputs.tags }}"; fi) \
-          --dry-run \
-          --ci \
-          --changed-since HEAD^) || {
-            echo "nf-test command failed with exit code $?"
-            echo "Full output: $nftest_output"
-            exit 1
-        }
-        echo "nf-test dry-run output: $nftest_output"
-
-        # Default values for shard and total_shards
-        shard="[]"
-        total_shards=0
-
-        # Check if there are related tests
-        if echo "$nftest_output" | grep -q 'No tests to execute'; then
-          echo "No related tests found."
-        else
-          # Extract the number of related tests
-          number_of_shards=$(echo "$nftest_output" | sed -n 's|.*Executed \([0-9]*\) tests.*|\1|p')
-          if [[ -n "$number_of_shards" && "$number_of_shards" -gt 0 ]]; then
-            shards_to_run=$(( $number_of_shards < ${{ inputs.max_shards }} ? $number_of_shards : ${{ inputs.max_shards }} ))
-            shard=$(seq 1 "$shards_to_run" | jq -R . | jq -c -s .)
-            total_shards="$shards_to_run"
-          else
-            echo "Unexpected output format. Falling back to default values."
-          fi
-        fi
-
-        # Write to GitHub Actions outputs
-        echo "shard=$shard" >> $GITHUB_OUTPUT
-        echo "total_shards=$total_shards" >> $GITHUB_OUTPUT
-
-        # Debugging output
-        echo "Final shard array: $shard"
-        echo "Total number of shards: $total_shards"
diff --git a/.github/actions/nf-test/action.yml b/.github/actions/nf-test/action.yml
deleted file mode 100644
index 3b9724c..0000000
--- a/.github/actions/nf-test/action.yml
+++ /dev/null
@@ -1,111 +0,0 @@
-name: "nf-test Action"
-description: "Runs nf-test with common setup steps"
-inputs:
-  profile:
-    description: "Profile to use"
-    required: true
-  shard:
-    description: "Shard number for this CI job"
-    required: true
-  total_shards:
-    description: "Total number of test shards(NOT the total number of matrix jobs)"
-    required: true
-  paths:
-    description: "Test paths"
-    required: true
-  tags:
-    description: "Tags to pass as argument for nf-test --tag parameter"
-    required: false
-runs:
-  using: "composite"
-  steps:
-    - name: Setup Nextflow
-      uses: nf-core/setup-nextflow@v2
-      with:
-        version: "${{ env.NXF_VERSION }}"
-
-    - name: Set up Python
-      uses: actions/setup-python@e797f83bcb11b83ae66e0230d6156d7c80228e7c # v6
-      with:
-        python-version: "3.14"
-
-    - name: Install nf-test
-      uses: nf-core/setup-nf-test@v1
-      with:
-        version: "${{ env.NFT_VER }}"
-        install-pdiff: true
-
-    - name: Setup apptainer
-      if: contains(inputs.profile, 'singularity')
-      uses: eWaterCycle/setup-apptainer@main
-
-    - name: Set up Singularity
-      if: contains(inputs.profile, 'singularity')
-      shell: bash
-      run: |
-        mkdir -p $NXF_SINGULARITY_CACHEDIR
-        mkdir -p $NXF_SINGULARITY_LIBRARYDIR
-
-    - name: Conda setup
-      if: contains(inputs.profile, 'conda')
-      uses: conda-incubator/setup-miniconda@505e6394dae86d6a5c7fbb6e3fb8938e3e863830 # v3
-      with:
-        auto-update-conda: true
-        conda-solver: libmamba
-        channels: conda-forge
-        channel-priority: strict
-        conda-remove-defaults: true
-
-    - name: Run nf-test
-      shell: bash
-      env:
-        NFT_WORKDIR: ${{ env.NFT_WORKDIR }}
-      run: |
-        nf-test test \
-          --profile=+${{ inputs.profile }} \
-          $(if [ -n "${{ inputs.tags }}" ]; then echo "--tag ${{ inputs.tags }}"; fi) \
-          --ci \
-          --changed-since HEAD^ \
-          --verbose \
-          --tap=test.tap \
-          --shard ${{ inputs.shard }}/${{ inputs.total_shards }}
-
-          # Save the absolute path of the test.tap file to the output
-          echo "tap_file_path=$(realpath test.tap)" >> $GITHUB_OUTPUT
-
-    - name: Generate test summary
-      if: always()
-      shell: bash
-      run: |
-        # Add header if it doesn't exist (using a token file to track this)
-        if [ ! -f ".summary_header" ]; then
-          echo "# 🚀 nf-test results" >> $GITHUB_STEP_SUMMARY
-          echo "" >> $GITHUB_STEP_SUMMARY
-          echo "| Status | Test Name | Profile | Shard |" >> $GITHUB_STEP_SUMMARY
-          echo "|:------:|-----------|---------|-------|" >> $GITHUB_STEP_SUMMARY
-          touch .summary_header
-        fi
-
-        if [ -f test.tap ]; then
-          while IFS= read -r line; do
-            if [[ $line =~ ^ok ]]; then
-              test_name="${line#ok }"
-              # Remove the test number from the beginning
-              test_name="${test_name#* }"
-              echo "| ✅ | ${test_name} | ${{ inputs.profile }} | ${{ inputs.shard }}/${{ inputs.total_shards }} |" >> $GITHUB_STEP_SUMMARY
-            elif [[ $line =~ ^not\ ok ]]; then
-              test_name="${line#not ok }"
-              # Remove the test number from the beginning
-              test_name="${test_name#* }"
-              echo "| ❌ | ${test_name} | ${{ inputs.profile }} | ${{ inputs.shard }}/${{ inputs.total_shards }} |" >> $GITHUB_STEP_SUMMARY
-            fi
-          done < test.tap
-        else
-          echo "| ⚠️ | No test results found | ${{ inputs.profile }} | ${{ inputs.shard }}/${{ inputs.total_shards }} |" >> $GITHUB_STEP_SUMMARY
-        fi
-
-    - name: Clean up
-      if: always()
-      shell: bash
-      run: |
-        sudo rm -rf /home/ubuntu/tests/
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index 04028fa..e47925c 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -4,23 +4,44 @@ name: nf-core AWS full size tests
 # It runs the -profile 'test_full' on AWS batch
 
 on:
+  pull_request:
+    branches:
+      - main
+      - master
   workflow_dispatch:
   pull_request_review:
     types: [submitted]
-  release:
-    types: [published]
 
 jobs:
   run-platform:
     name: Run AWS full tests
-    # run only if the PR is approved by at least 2 reviewers and against the master/main branch or manually triggered
-    if: github.repository == 'nf-core/deepmutscan' && github.event.review.state == 'approved' && (github.event.pull_request.base.ref == 'master' || github.event.pull_request.base.ref == 'main') || github.event_name == 'workflow_dispatch' || github.event_name == 'release'
+    # run only if the PR is approved by at least 2 reviewers and against the master branch or manually triggered
+    if: github.repository == 'nf-core/deepmutscan' && github.event.review.state == 'approved' && github.event.pull_request.base.ref == 'master' || github.event_name == 'workflow_dispatch'
     runs-on: ubuntu-latest
     steps:
-      - name: Set revision variable
-        id: revision
+      - name: Get PR reviews
+        uses: octokit/request-action@v2.x
+        if: github.event_name != 'workflow_dispatch'
+        id: check_approvals
+        continue-on-error: true
+        with:
+          route: GET /repos/${{ github.repository }}/pulls/${{ github.event.pull_request.number }}/reviews?per_page=100
+        env:
+          GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
+
+      - name: Check for approvals
+        if: ${{ failure() && github.event_name != 'workflow_dispatch' }}
+        run: |
+          echo "No review approvals found. At least 2 approvals are required to run this action automatically."
+          exit 1
+
+      - name: Check for enough approvals (>=2)
+        id: test_variables
+        if: github.event_name != 'workflow_dispatch'
         run: |
-          echo "revision=${{ (github.event_name == 'workflow_dispatch' || github.event_name == 'release') && github.sha || 'dev' }}" >> "$GITHUB_OUTPUT"
+          JSON_RESPONSE='${{ steps.check_approvals.outputs.data }}'
+          CURRENT_APPROVALS_COUNT=$(echo $JSON_RESPONSE | jq -c '[.[] | select(.state | contains("APPROVED")) ] | length')
+          test $CURRENT_APPROVALS_COUNT -ge 2 || exit 1 # At least 2 approvals are required
 
       - name: Launch workflow via Seqera Platform
         uses: seqeralabs/action-tower-launch@v2
@@ -28,21 +49,21 @@ jobs:
         # Add full size test data (but still relatively small datasets for few samples)
         # on the `test_full.config` test runs with only one set of parameters
         with:
-          workspace_id: ${{ vars.TOWER_WORKSPACE_ID }}
+          workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
-          compute_env: ${{ vars.TOWER_COMPUTE_ENV }}
-          revision: ${{ steps.revision.outputs.revision }}
-          workdir: s3://${{ vars.AWS_S3_BUCKET }}/work/deepmutscan/work-${{ steps.revision.outputs.revision }}
+          compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
+          revision: ${{ github.sha }}
+          workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/deepmutscan/work-${{ github.sha }}
           parameters: |
             {
               "hook_url": "${{ secrets.MEGATESTS_ALERTS_SLACK_HOOK_URL }}",
-              "outdir": "s3://${{ vars.AWS_S3_BUCKET }}/deepmutscan/results-${{ steps.revision.outputs.revision }}"
+              "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/deepmutscan/results-${{ github.sha }}"
             }
           profiles: test_full
 
-      - uses: actions/upload-artifact@330a01c490aca151604b8cf639adc76d48f6c5d4 # v5
+      - uses: actions/upload-artifact@v4
         with:
           name: Seqera Platform debug log file
           path: |
-            tower_action_*.log
-            tower_action_*.json
+            seqera_platform_action_*.log
+            seqera_platform_action_*.json
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
index 9e687d3..0f92edd 100644
--- a/.github/workflows/awstest.yml
+++ b/.github/workflows/awstest.yml
@@ -14,20 +14,20 @@ jobs:
       - name: Launch workflow via Seqera Platform
         uses: seqeralabs/action-tower-launch@v2
         with:
-          workspace_id: ${{ vars.TOWER_WORKSPACE_ID }}
+          workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
           access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
-          compute_env: ${{ vars.TOWER_COMPUTE_ENV }}
+          compute_env: ${{ secrets.TOWER_COMPUTE_ENV }}
           revision: ${{ github.sha }}
-          workdir: s3://${{ vars.AWS_S3_BUCKET }}/work/deepmutscan/work-${{ github.sha }}
+          workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/deepmutscan/work-${{ github.sha }}
           parameters: |
             {
-              "outdir": "s3://${{ vars.AWS_S3_BUCKET }}/deepmutscan/results-test-${{ github.sha }}"
+              "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/deepmutscan/results-test-${{ github.sha }}"
             }
           profiles: test
 
-      - uses: actions/upload-artifact@330a01c490aca151604b8cf639adc76d48f6c5d4 # v5
+      - uses: actions/upload-artifact@v4
         with:
           name: Seqera Platform debug log file
           path: |
-            tower_action_*.log
-            tower_action_*.json
+            seqera_platform_action_*.log
+            seqera_platform_action_*.json
diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
new file mode 100644
index 0000000..c23beda
--- /dev/null
+++ b/.github/workflows/ci.yml
@@ -0,0 +1,87 @@
+name: nf-core CI
+# This workflow runs the pipeline with the minimal test dataset to check that it completes without any syntax errors
+on:
+  push:
+    branches:
+      - dev
+  pull_request:
+  release:
+    types: [published]
+  workflow_dispatch:
+
+env:
+  NXF_ANSI_LOG: false
+  NXF_SINGULARITY_CACHEDIR: ${{ github.workspace }}/.singularity
+  NXF_SINGULARITY_LIBRARYDIR: ${{ github.workspace }}/.singularity
+
+concurrency:
+  group: "${{ github.workflow }}-${{ github.event.pull_request.number || github.ref }}"
+  cancel-in-progress: true
+
+jobs:
+  test:
+    name: "Run pipeline with test data (${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }})"
+    # Only run on push if this is the nf-core dev branch (merged PRs)
+    if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/deepmutscan') }}"
+    runs-on: ubuntu-latest
+    strategy:
+      matrix:
+        NXF_VER:
+          - "24.04.2"
+          - "latest-everything"
+        profile:
+          - "conda"
+          - "docker"
+          - "singularity"
+        test_name:
+          - "test"
+        isMaster:
+          - ${{ github.base_ref == 'master' }}
+        # Exclude conda and singularity on dev
+        exclude:
+          - isMaster: false
+            profile: "conda"
+          - isMaster: false
+            profile: "singularity"
+    steps:
+      - name: Check out pipeline code
+        uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
+        with:
+          fetch-depth: 0
+
+      - name: Set up Nextflow
+        uses: nf-core/setup-nextflow@v2
+        with:
+          version: "${{ matrix.NXF_VER }}"
+
+      - name: Set up Apptainer
+        if: matrix.profile == 'singularity'
+        uses: eWaterCycle/setup-apptainer@main
+
+      - name: Set up Singularity
+        if: matrix.profile == 'singularity'
+        run: |
+          mkdir -p $NXF_SINGULARITY_CACHEDIR
+          mkdir -p $NXF_SINGULARITY_LIBRARYDIR
+
+      - name: Set up Miniconda
+        if: matrix.profile == 'conda'
+        uses: conda-incubator/setup-miniconda@a4260408e20b96e80095f42ff7f1a15b27dd94ca # v3
+        with:
+          miniconda-version: "latest"
+          auto-update-conda: true
+          conda-solver: libmamba
+          channels: conda-forge,bioconda
+
+      - name: Set up Conda
+        if: matrix.profile == 'conda'
+        run: |
+          echo $(realpath $CONDA)/condabin >> $GITHUB_PATH
+          echo $(realpath python) >> $GITHUB_PATH
+
+      - name: Clean up Disk space
+        uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
+
+      - name: "Run pipeline with test data ${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }}"
+        run: |
+          nextflow run ${GITHUB_WORKSPACE} -profile ${{ matrix.test_name }},${{ matrix.profile }} --outdir ./results
diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml
index 6adb0ff..0b6b1f2 100644
--- a/.github/workflows/clean-up.yml
+++ b/.github/workflows/clean-up.yml
@@ -10,7 +10,7 @@ jobs:
       issues: write
       pull-requests: write
     steps:
-      - uses: actions/stale@5f858e3efba33a5ca4407a664cc011ad407f2008 # v10
+      - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9
         with:
           stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days."
           stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful."
diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml
index 45884ff..ab06316 100644
--- a/.github/workflows/download_pipeline.yml
+++ b/.github/workflows/download_pipeline.yml
@@ -12,6 +12,14 @@ on:
         required: true
         default: "dev"
   pull_request:
+    types:
+      - opened
+      - edited
+      - synchronize
+    branches:
+      - main
+      - master
+  pull_request_target:
     branches:
       - main
       - master
@@ -44,9 +52,9 @@ jobs:
       - name: Disk space cleanup
         uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
 
-      - uses: actions/setup-python@e797f83bcb11b83ae66e0230d6156d7c80228e7c # v6
+      - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
         with:
-          python-version: "3.14"
+          python-version: "3.12"
           architecture: "x64"
 
       - name: Setup Apptainer
@@ -57,7 +65,7 @@ jobs:
       - name: Install dependencies
         run: |
           python -m pip install --upgrade pip
-          pip install git+https://github.com/nf-core/tools.git
+          pip install git+https://github.com/nf-core/tools.git@dev
 
       - name: Make a cache directory for the container images
         run: |
@@ -112,7 +120,6 @@ jobs:
           echo "IMAGE_COUNT_AFTER=$image_count" >> "$GITHUB_OUTPUT"
 
       - name: Compare container image counts
-        id: count_comparison
         run: |
           if [ "${{ steps.count_initial.outputs.IMAGE_COUNT_INITIAL }}" -ne "${{ steps.count_afterwards.outputs.IMAGE_COUNT_AFTER }}" ]; then
             initial_count=${{ steps.count_initial.outputs.IMAGE_COUNT_INITIAL }}
@@ -125,10 +132,3 @@ jobs:
           else
             echo "The pipeline can be downloaded successfully!"
           fi
-
-      - name: Upload Nextflow logfile for debugging purposes
-        uses: actions/upload-artifact@330a01c490aca151604b8cf639adc76d48f6c5d4 # v5
-        with:
-          name: nextflow_logfile.txt
-          path: .nextflow.log*
-          include-hidden-files: true
diff --git a/.github/workflows/fix_linting.yml b/.github/workflows/fix-linting.yml
similarity index 80%
rename from .github/workflows/fix_linting.yml
rename to .github/workflows/fix-linting.yml
index ea03315..8bda64e 100644
--- a/.github/workflows/fix_linting.yml
+++ b/.github/workflows/fix-linting.yml
@@ -13,13 +13,13 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       # Use the @nf-core-bot token to check out so we can push later
-      - uses: actions/checkout@93cb6efe18208431cddfb8368fd83d5badbf9bfd # v5
+      - uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
         with:
           token: ${{ secrets.nf_core_bot_auth_token }}
 
       # indication that the linting is being fixed
       - name: React on comment
-        uses: peter-evans/create-or-update-comment@e8674b075228eee787fea43ef493e45ece1004c9 # v5
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
         with:
           comment-id: ${{ github.event.comment.id }}
           reactions: eyes
@@ -32,9 +32,9 @@ jobs:
           GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }}
 
       # Install and run pre-commit
-      - uses: actions/setup-python@e797f83bcb11b83ae66e0230d6156d7c80228e7c # v6
+      - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
         with:
-          python-version: "3.14"
+          python-version: "3.12"
 
       - name: Install pre-commit
         run: pip install pre-commit
@@ -47,7 +47,7 @@ jobs:
       # indication that the linting has finished
       - name: react if linting finished succesfully
         if: steps.pre-commit.outcome == 'success'
-        uses: peter-evans/create-or-update-comment@e8674b075228eee787fea43ef493e45ece1004c9 # v5
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
         with:
           comment-id: ${{ github.event.comment.id }}
           reactions: "+1"
@@ -67,21 +67,21 @@ jobs:
       - name: react if linting errors were fixed
         id: react-if-fixed
         if: steps.commit-and-push.outcome == 'success'
-        uses: peter-evans/create-or-update-comment@e8674b075228eee787fea43ef493e45ece1004c9 # v5
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
         with:
           comment-id: ${{ github.event.comment.id }}
           reactions: hooray
 
       - name: react if linting errors were not fixed
         if: steps.commit-and-push.outcome == 'failure'
-        uses: peter-evans/create-or-update-comment@e8674b075228eee787fea43ef493e45ece1004c9 # v5
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
         with:
           comment-id: ${{ github.event.comment.id }}
           reactions: confused
 
       - name: react if linting errors were not fixed
         if: steps.commit-and-push.outcome  == 'failure'
-        uses: peter-evans/create-or-update-comment@e8674b075228eee787fea43ef493e45ece1004c9 # v5
+        uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4
         with:
           issue-number: ${{ github.event.issue.number }}
           body: |
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 7a527a3..dbd52d5 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -3,6 +3,9 @@ name: nf-core linting
 # It runs the `nf-core pipelines lint` and markdown lint tests to ensure
 # that the code meets the nf-core guidelines.
 on:
+  push:
+    branches:
+      - dev
   pull_request:
   release:
     types: [published]
@@ -11,12 +14,12 @@ jobs:
   pre-commit:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@93cb6efe18208431cddfb8368fd83d5badbf9bfd # v5
+      - uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
 
-      - name: Set up Python 3.14
-        uses: actions/setup-python@e797f83bcb11b83ae66e0230d6156d7c80228e7c # v6
+      - name: Set up Python 3.12
+        uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
         with:
-          python-version: "3.14"
+          python-version: "3.12"
 
       - name: Install pre-commit
         run: pip install pre-commit
@@ -28,18 +31,18 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@93cb6efe18208431cddfb8368fd83d5badbf9bfd # v5
+        uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
 
       - name: Install Nextflow
         uses: nf-core/setup-nextflow@v2
 
-      - uses: actions/setup-python@e797f83bcb11b83ae66e0230d6156d7c80228e7c # v6
+      - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
         with:
-          python-version: "3.14"
+          python-version: "3.12"
           architecture: "x64"
 
       - name: read .nf-core.yml
-        uses: pietrobolcato/action-read-yaml@9f13718d61111b69f30ab4ac683e67a56d254e1d # 1.1.0
+        uses: pietrobolcato/action-read-yaml@1.1.0
         id: read_yml
         with:
           config: ${{ github.workspace }}/.nf-core.yml
@@ -71,7 +74,7 @@ jobs:
 
       - name: Upload linting log file artifact
         if: ${{ always() }}
-        uses: actions/upload-artifact@330a01c490aca151604b8cf639adc76d48f6c5d4 # v5
+        uses: actions/upload-artifact@b4b15b8c7c6ac21ea08fcf65892d2ee8f75cf882 # v4
         with:
           name: linting-logs
           path: |
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index e6e9bc2..0bed96d 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -11,7 +11,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Download lint results
-        uses: dawidd6/action-download-artifact@ac66b43f0e6a346234dd65d4d0c8fbb31cb316e5 # v11
+        uses: dawidd6/action-download-artifact@80620a5d27ce0ae443b965134db88467fc607b43 # v7
         with:
           workflow: linting.yml
           workflow_conclusion: completed
@@ -21,7 +21,7 @@ jobs:
         run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT
 
       - name: Post PR comment
-        uses: marocchino/sticky-pull-request-comment@773744901bac0e8cbb5a0dc842800d45e9b2b405 # v2
+        uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2
         with:
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           number: ${{ steps.pr_number.outputs.pr_number }}
diff --git a/.github/workflows/nf-test.yml b/.github/workflows/nf-test.yml
deleted file mode 100644
index c98d76e..0000000
--- a/.github/workflows/nf-test.yml
+++ /dev/null
@@ -1,144 +0,0 @@
-name: Run nf-test
-on:
-  pull_request:
-    paths-ignore:
-      - "docs/**"
-      - "**/meta.yml"
-      - "**/*.md"
-      - "**/*.png"
-      - "**/*.svg"
-  release:
-    types: [published]
-  workflow_dispatch:
-
-# Cancel if a newer run is started
-concurrency:
-  group: ${{ github.workflow }}-${{ github.event.pull_request.number || github.ref }}
-  cancel-in-progress: true
-
-env:
-  GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
-  NFT_VER: "0.9.3"
-  NFT_WORKDIR: "~"
-  NXF_ANSI_LOG: false
-  NXF_SINGULARITY_CACHEDIR: ${{ github.workspace }}/.singularity
-  NXF_SINGULARITY_LIBRARYDIR: ${{ github.workspace }}/.singularity
-
-jobs:
-  nf-test-changes:
-    name: nf-test-changes
-    runs-on: # use self-hosted runners
-      - runs-on=${{ github.run_id }}-nf-test-changes
-      - runner=4cpu-linux-x64
-    outputs:
-      shard: ${{ steps.set-shards.outputs.shard }}
-      total_shards: ${{ steps.set-shards.outputs.total_shards }}
-    steps:
-      - name: Clean Workspace # Purge the workspace in case it's running on a self-hosted runner
-        run: |
-          ls -la ./
-          rm -rf ./* || true
-          rm -rf ./.??* || true
-          ls -la ./
-      - uses: actions/checkout@93cb6efe18208431cddfb8368fd83d5badbf9bfd # v5
-        with:
-          fetch-depth: 0
-
-      - name: get number of shards
-        id: set-shards
-        uses: ./.github/actions/get-shards
-        env:
-          NFT_VER: ${{ env.NFT_VER }}
-        with:
-          max_shards: 7
-
-      - name: debug
-        run: |
-          echo ${{ steps.set-shards.outputs.shard }}
-          echo ${{ steps.set-shards.outputs.total_shards }}
-
-  nf-test:
-    name: "${{ matrix.profile }} | ${{ matrix.NXF_VER }} | ${{ matrix.shard }}/${{ needs.nf-test-changes.outputs.total_shards }}"
-    needs: [nf-test-changes]
-    if: ${{ needs.nf-test-changes.outputs.total_shards != '0' }}
-    runs-on: # use self-hosted runners
-      - runs-on=${{ github.run_id }}-nf-test
-      - runner=4cpu-linux-x64
-    strategy:
-      fail-fast: false
-      matrix:
-        shard: ${{ fromJson(needs.nf-test-changes.outputs.shard) }}
-        profile: [conda, docker, singularity]
-        isMain:
-          - ${{ github.base_ref == 'master' || github.base_ref == 'main' }}
-        # Exclude conda and singularity on dev
-        exclude:
-          - isMain: false
-            profile: "conda"
-          - isMain: false
-            profile: "singularity"
-        NXF_VER:
-          - "25.04.0"
-          - "latest-everything"
-    env:
-      NXF_ANSI_LOG: false
-      TOTAL_SHARDS: ${{ needs.nf-test-changes.outputs.total_shards }}
-
-    steps:
-      - uses: actions/checkout@93cb6efe18208431cddfb8368fd83d5badbf9bfd # v5
-        with:
-          fetch-depth: 0
-
-      - name: Run nf-test
-        id: run_nf_test
-        uses: ./.github/actions/nf-test
-        continue-on-error: ${{ matrix.NXF_VER == 'latest-everything' }}
-        env:
-          NFT_WORKDIR: ${{ env.NFT_WORKDIR }}
-          NXF_VERSION: ${{ matrix.NXF_VER }}
-        with:
-          profile: ${{ matrix.profile }}
-          shard: ${{ matrix.shard }}
-          total_shards: ${{ env.TOTAL_SHARDS }}
-
-      - name: Report test status
-        if: ${{ always() }}
-        run: |
-          if [[ "${{ steps.run_nf_test.outcome }}" == "failure" ]]; then
-            echo "::error::Test with ${{ matrix.NXF_VER }} failed"
-            # Add to workflow summary
-            echo "## ❌ Test failed: ${{ matrix.profile }} | ${{ matrix.NXF_VER }} | Shard ${{ matrix.shard }}/${{ env.TOTAL_SHARDS }}" >> $GITHUB_STEP_SUMMARY
-            if [[ "${{ matrix.NXF_VER }}" == "latest-everything" ]]; then
-              echo "::warning::Test with latest-everything failed but will not cause workflow failure. Please check if the error is expected or if it needs fixing."
-            fi
-            if [[ "${{ matrix.NXF_VER }}" != "latest-everything" ]]; then
-              exit 1
-            fi
-          fi
-
-  confirm-pass:
-    needs: [nf-test]
-    if: always()
-    runs-on: # use self-hosted runners
-      - runs-on=${{ github.run_id }}-confirm-pass
-      - runner=2cpu-linux-x64
-    steps:
-      - name: One or more tests failed (excluding latest-everything)
-        if: ${{ contains(needs.*.result, 'failure') }}
-        run: exit 1
-
-      - name: One or more tests cancelled
-        if: ${{ contains(needs.*.result, 'cancelled') }}
-        run: exit 1
-
-      - name: All tests ok
-        if: ${{ contains(needs.*.result, 'success') }}
-        run: exit 0
-
-      - name: debug-print
-        if: always()
-        run: |
-          echo "::group::DEBUG: `needs` Contents"
-          echo "DEBUG: toJSON(needs) = ${{ toJSON(needs) }}"
-          echo "DEBUG: toJSON(needs.*.result) = ${{ toJSON(needs.*.result) }}"
-          echo "::endgroup::"
diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml
index 431d3d4..450b1d5 100644
--- a/.github/workflows/release-announcements.yml
+++ b/.github/workflows/release-announcements.yml
@@ -14,10 +14,6 @@ jobs:
         run: |
           echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')" | sed 's/-//g' >> $GITHUB_OUTPUT
 
-      - name: get description
-        id: get_description
-        run: |
-          echo "description=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .description')" >> $GITHUB_OUTPUT
       - uses: rzr/fediverse-action@master
         with:
           access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }}
@@ -26,15 +22,48 @@ jobs:
           # https://docs.github.com/en/developers/webhooks-and-events/webhooks/webhook-events-and-payloads#release
           message: |
             Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}!
-            ${{ steps.get_description.outputs.description }}
+
             Please see the changelog: ${{ github.event.release.html_url }}
 
             ${{ steps.get_topics.outputs.topics }} #nfcore #openscience #nextflow #bioinformatics
 
+  send-tweet:
+    runs-on: ubuntu-latest
+
+    steps:
+      - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
+        with:
+          python-version: "3.10"
+      - name: Install dependencies
+        run: pip install tweepy==4.14.0
+      - name: Send tweet
+        shell: python
+        run: |
+          import os
+          import tweepy
+
+          client = tweepy.Client(
+              access_token=os.getenv("TWITTER_ACCESS_TOKEN"),
+              access_token_secret=os.getenv("TWITTER_ACCESS_TOKEN_SECRET"),
+              consumer_key=os.getenv("TWITTER_CONSUMER_KEY"),
+              consumer_secret=os.getenv("TWITTER_CONSUMER_SECRET"),
+          )
+          tweet = os.getenv("TWEET")
+          client.create_tweet(text=tweet)
+        env:
+          TWEET: |
+            Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}!
+
+            Please see the changelog: ${{ github.event.release.html_url }}
+          TWITTER_CONSUMER_KEY: ${{ secrets.TWITTER_CONSUMER_KEY }}
+          TWITTER_CONSUMER_SECRET: ${{ secrets.TWITTER_CONSUMER_SECRET }}
+          TWITTER_ACCESS_TOKEN: ${{ secrets.TWITTER_ACCESS_TOKEN }}
+          TWITTER_ACCESS_TOKEN_SECRET: ${{ secrets.TWITTER_ACCESS_TOKEN_SECRET }}
+
   bsky-post:
     runs-on: ubuntu-latest
     steps:
-      - uses: zentered/bluesky-post-action@6461056ea355ea43b977e149f7bf76aaa572e5e8 # v0.3.0
+      - uses: zentered/bluesky-post-action@80dbe0a7697de18c15ad22f4619919ceb5ccf597 # v0.1.0
         with:
           post: |
             Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}!
diff --git a/.github/workflows/template-version-comment.yml b/.github/workflows/template_version_comment.yml
similarity index 91%
rename from .github/workflows/template-version-comment.yml
rename to .github/workflows/template_version_comment.yml
index e8560fc..537529b 100644
--- a/.github/workflows/template-version-comment.yml
+++ b/.github/workflows/template_version_comment.yml
@@ -9,12 +9,12 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@93cb6efe18208431cddfb8368fd83d5badbf9bfd # v5
+        uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
         with:
           ref: ${{ github.event.pull_request.head.sha }}
 
       - name: Read template version from .nf-core.yml
-        uses: nichmor/minimal-read-yaml@1f7205277e25e156e1f63815781db80a6d490b8f # v0.0.2
+        uses: nichmor/minimal-read-yaml@v0.0.2
         id: read_yml
         with:
           config: ${{ github.workspace }}/.nf-core.yml
diff --git a/.gitpod.yml b/.gitpod.yml
new file mode 100644
index 0000000..83599f6
--- /dev/null
+++ b/.gitpod.yml
@@ -0,0 +1,10 @@
+image: nfcore/gitpod:latest
+tasks:
+  - name: Update Nextflow and setup pre-commit
+    command: |
+      pre-commit install --install-hooks
+      nextflow self-update
+
+vscode:
+  extensions:
+    - nf-core.nf-core-extensionpack # https://github.com/nf-core/vscode-extensionpack
diff --git a/.nf-core.yml b/.nf-core.yml
index a24b80a..f23f386 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -1,5 +1,5 @@
 lint: {}
-nf_core_version: 3.5.1
+nf_core_version: 3.1.2
 repository_type: pipeline
 template:
   author: Benjamin Wehnert & Max Stammnitz
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index d06777a..9e9f0e1 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -4,24 +4,10 @@ repos:
     hooks:
       - id: prettier
         additional_dependencies:
-          - prettier@3.6.2
-  - repo: https://github.com/pre-commit/pre-commit-hooks
-    rev: v6.0.0
+          - prettier@3.2.5
+
+  - repo: https://github.com/editorconfig-checker/editorconfig-checker.python
+    rev: "3.0.3"
     hooks:
-      - id: trailing-whitespace
-        args: [--markdown-linebreak-ext=md]
-        exclude: |
-          (?x)^(
-              .*ro-crate-metadata.json$|
-              modules/nf-core/.*|
-              subworkflows/nf-core/.*|
-              .*\.snap$
-          )$
-      - id: end-of-file-fixer
-        exclude: |
-          (?x)^(
-              .*ro-crate-metadata.json$|
-              modules/nf-core/.*|
-              subworkflows/nf-core/.*|
-              .*\.snap$
-          )$
+      - id: editorconfig-checker
+        alias: ec
diff --git a/.prettierignore b/.prettierignore
index dd749d4..edd29f0 100644
--- a/.prettierignore
+++ b/.prettierignore
@@ -10,7 +10,4 @@ testing/
 testing*
 *.pyc
 bin/
-.nf-test/
 ro-crate-metadata.json
-modules/nf-core/
-subworkflows/nf-core/
diff --git a/.prettierrc.yml b/.prettierrc.yml
index 07dbd8b..c81f9a7 100644
--- a/.prettierrc.yml
+++ b/.prettierrc.yml
@@ -1,6 +1 @@
 printWidth: 120
-tabWidth: 4
-overrides:
-  - files: "*.{md,yml,yaml,html,css,scss,js,cff}"
-    options:
-      tabWidth: 2
diff --git a/README.md b/README.md
index 4e8dc52..3898d62 100644
--- a/README.md
+++ b/README.md
@@ -5,19 +5,17 @@
   </picture>
 </h1>
 
-[![Open in GitHub Codespaces](https://img.shields.io/badge/Open_In_GitHub_Codespaces-black?labelColor=grey&logo=github)](https://github.com/codespaces/new/nf-core/deepmutscan)
-[![GitHub Actions CI Status](https://github.com/nf-core/deepmutscan/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/deepmutscan/actions/workflows/nf-test.yml)
+[![GitHub Actions CI Status](https://github.com/nf-core/deepmutscan/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/deepmutscan/actions/workflows/ci.yml)
 [![GitHub Actions Linting Status](https://github.com/nf-core/deepmutscan/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/deepmutscan/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/deepmutscan/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)
 [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)
 
-[![Nextflow](https://img.shields.io/badge/version-%E2%89%A525.04.0-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)
-[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.5.1-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.5.1)
+[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
 [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
 [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
 [![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/deepmutscan)
 
-[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23deepmutscan-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/deepmutscan)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)
+[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23deepmutscan-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/deepmutscan)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)
 
 ## Introduction
 
@@ -30,7 +28,7 @@
 -->
 
 <!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core
-     workflows use the "tube map" design for that. See https://nf-co.re/docs/guidelines/graphic_design/workflow_diagrams#examples for examples.   -->
+     workflows use the "tube map" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples.   -->
 <!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
 
 ## Usage
diff --git a/assets/nf-core-deepmutscan_logo_light.png b/assets/nf-core-deepmutscan_logo_light.png
index d6c8e55be2c4776dd4e422bec64f95bd4fc97739..9824bb2d087a68cc4b3801ded12e74fee21dab13 100644
GIT binary patch
delta 47419
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diff --git a/assets/schema_input.json b/assets/schema_input.json
index 656b88f..062831a 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -17,14 +17,14 @@
                 "type": "string",
                 "format": "file-path",
                 "exists": true,
-                "pattern": "^([\\S\\s]*\\/)?[^\\s\\/]+\\.f(ast)?q\\.gz$",
+                "pattern": "^\\S+\\.f(ast)?q\\.gz$",
                 "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'"
             },
             "fastq_2": {
                 "type": "string",
                 "format": "file-path",
                 "exists": true,
-                "pattern": "^([\\S\\s]*\\/)?[^\\s\\/]+\\.f(ast)?q\\.gz$",
+                "pattern": "^\\S+\\.f(ast)?q\\.gz$",
                 "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'"
             }
         },
diff --git a/conf/base.config b/conf/base.config
index 248f24c..e4e941d 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -15,7 +15,7 @@ process {
     memory = { 6.GB   * task.attempt }
     time   = { 4.h    * task.attempt }
 
-    errorStrategy = { task.exitStatus in ((130..145) + 104 + 175) ? 'retry' : 'finish' }
+    errorStrategy = { task.exitStatus in ((130..145) + 104) ? 'retry' : 'finish' }
     maxRetries    = 1
     maxErrors     = '-1'
 
@@ -59,8 +59,4 @@ process {
         errorStrategy = 'retry'
         maxRetries    = 2
     }
-    withLabel: process_gpu {
-        ext.use_gpu = { workflow.profile.contains('gpu') }
-        accelerator = { workflow.profile.contains('gpu') ? 1 : null }
-    }
 }
diff --git a/docs/images/nf-core-deepmutscan_logo_dark.png b/docs/images/nf-core-deepmutscan_logo_dark.png
index 398e0b2c3f7043c2781d2d83a0f9fe2fbe3e064a..8b387faba425d60bebf7bafaf89552133aa33485 100644
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diff --git a/docs/output.md b/docs/output.md
index 743e91f..2db7739 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -12,8 +12,7 @@ The directories listed below will be created in the results directory after the
 
 The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps:
 
-- [FastQC](#fastqc) - Raw read QC
-- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
+- [FastQC](#fastqc) - Raw read QC- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
 - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution
 
 ### FastQC
@@ -27,9 +26,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 
 </details>
 
-[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/).
-
-### MultiQC
+[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/).### MultiQC
 
 <details markdown="1">
 <summary>Output files</summary>
@@ -43,9 +40,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
 
 [MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory.
 
-Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see <http://multiqc.info>.
-
-### Pipeline information
+Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see <http://multiqc.info>.### Pipeline information
 
 <details markdown="1">
 <summary>Output files</summary>
diff --git a/docs/usage.md b/docs/usage.md
index e7605a3..6c1fddc 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -149,7 +149,7 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof
 - `shifter`
   - A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/)
 - `charliecloud`
-  - A generic configuration profile to be used with [Charliecloud](https://charliecloud.io/)
+  - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/)
 - `apptainer`
   - A generic configuration profile to be used with [Apptainer](https://apptainer.org/)
 - `wave`
diff --git a/main.nf b/main.nf
index 1497039..d668cea 100644
--- a/main.nf
+++ b/main.nf
@@ -74,10 +74,7 @@ workflow {
         params.monochrome_logs,
         args,
         params.outdir,
-        params.input,
-        params.help,
-        params.help_full,
-        params.show_hidden
+        params.input
     )
 
     //
diff --git a/modules.json b/modules.json
index efc56c9..e30d1fa 100644
--- a/modules.json
+++ b/modules.json
@@ -7,12 +7,12 @@
                 "nf-core": {
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "git_sha": "dc94b6ee04a05ddb9f7ae050712ff30a13149164",
                         "installed_by": ["modules"]
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "af27af1be706e6a2bb8fe454175b0cdf77f47b49",
+                        "git_sha": "cf17ca47590cc578dfb47db1c2a44ef86f89976d",
                         "installed_by": ["modules"]
                     }
                 }
@@ -21,17 +21,17 @@
                 "nf-core": {
                     "utils_nextflow_pipeline": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "c2b22d85f30a706a3073387f30380704fcae013b",
                         "installed_by": ["subworkflows"]
                     },
                     "utils_nfcore_pipeline": {
                         "branch": "master",
-                        "git_sha": "271e7fc14eb1320364416d996fb077421f3faed2",
+                        "git_sha": "51ae5406a030d4da1e49e4dab49756844fdd6c7a",
                         "installed_by": ["subworkflows"]
                     },
                     "utils_nfschema_plugin": {
                         "branch": "master",
-                        "git_sha": "4b406a74dc0449c0401ed87d5bfff4252fd277fd",
+                        "git_sha": "2fd2cd6d0e7b273747f32e465fdc6bcc3ae0814e",
                         "installed_by": ["subworkflows"]
                     }
                 }
diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml
index f9f54ee..691d4c7 100644
--- a/modules/nf-core/fastqc/environment.yml
+++ b/modules/nf-core/fastqc/environment.yml
@@ -1,5 +1,3 @@
----
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index 23e1663..752c3a1 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -1,5 +1,5 @@
 process FASTQC {
-    tag "${meta.id}"
+    tag "$meta.id"
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
@@ -19,30 +19,30 @@ process FASTQC {
     task.ext.when == null || task.ext.when
 
     script:
-    def args          = task.ext.args ?: ''
-    def prefix        = task.ext.prefix ?: "${meta.id}"
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
     // Make list of old name and new name pairs to use for renaming in the bash while loop
     def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] }
-    def rename_to     = old_new_pairs*.join(' ').join(' ')
+    def rename_to = old_new_pairs*.join(' ').join(' ')
     def renamed_files = old_new_pairs.collect{ _old_name, new_name -> new_name }.join(' ')
 
     // The total amount of allocated RAM by FastQC is equal to the number of threads defined (--threads) time the amount of RAM defined (--memory)
     // https://github.com/s-andrews/FastQC/blob/1faeea0412093224d7f6a07f777fad60a5650795/fastqc#L211-L222
     // Dividing the task.memory by task.cpu allows to stick to requested amount of RAM in the label
-    def memory_in_mb = task.memory ? task.memory.toUnit('MB') / task.cpus : null
+    def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') / task.cpus
     // FastQC memory value allowed range (100 - 10000)
     def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb)
 
     """
-    printf "%s %s\\n" ${rename_to} | while read old_name new_name; do
+    printf "%s %s\\n" $rename_to | while read old_name new_name; do
         [ -f "\${new_name}" ] || ln -s \$old_name \$new_name
     done
 
     fastqc \\
-        ${args} \\
-        --threads ${task.cpus} \\
-        --memory ${fastqc_memory} \\
-        ${renamed_files}
+        $args \\
+        --threads $task.cpus \\
+        --memory $fastqc_memory \\
+        $renamed_files
 
     cat <<-END_VERSIONS > versions.yml
     "${task.process}":
diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml
index c8d9d02..2b2e62b 100644
--- a/modules/nf-core/fastqc/meta.yml
+++ b/modules/nf-core/fastqc/meta.yml
@@ -29,10 +29,9 @@ input:
         description: |
           List of input FastQ files of size 1 and 2 for single-end and paired-end data,
           respectively.
-        ontologies: []
 output:
-  html:
-    - - meta:
+  - html:
+      - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -41,9 +40,8 @@ output:
           type: file
           description: FastQC report
           pattern: "*_{fastqc.html}"
-          ontologies: []
-  zip:
-    - - meta:
+  - zip:
+      - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -52,14 +50,11 @@ output:
           type: file
           description: FastQC report archive
           pattern: "*_{fastqc.zip}"
-          ontologies: []
-  versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@drpatelh"
   - "@grst"
diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml
new file mode 100644
index 0000000..7834294
--- /dev/null
+++ b/modules/nf-core/fastqc/tests/tags.yml
@@ -0,0 +1,2 @@
+fastqc:
+  - modules/nf-core/fastqc/**
diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml
index d02016a..6f5b867 100644
--- a/modules/nf-core/multiqc/environment.yml
+++ b/modules/nf-core/multiqc/environment.yml
@@ -1,7 +1,5 @@
----
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
 channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::multiqc=1.32
+  - bioconda::multiqc=1.25.1
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index c1158fb..cc0643e 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -3,8 +3,8 @@ process MULTIQC {
 
     conda "${moduleDir}/environment.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/8c/8c6c120d559d7ee04c7442b61ad7cf5a9e8970be5feefb37d68eeaa60c1034eb/data' :
-        'community.wave.seqera.io/library/multiqc:1.32--d58f60e4deb769bf' }"
+        'https://depot.galaxyproject.org/singularity/multiqc:1.25.1--pyhdfd78af_0' :
+        'biocontainers/multiqc:1.25.1--pyhdfd78af_0' }"
 
     input:
     path  multiqc_files, stageAs: "?/*"
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
index ce30eb7..b16c187 100644
--- a/modules/nf-core/multiqc/meta.yml
+++ b/modules/nf-core/multiqc/meta.yml
@@ -15,71 +15,57 @@ tools:
       licence: ["GPL-3.0-or-later"]
       identifier: biotools:multiqc
 input:
-  - multiqc_files:
-      type: file
-      description: |
-        List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
-      ontologies: []
-  - multiqc_config:
-      type: file
-      description: Optional config yml for MultiQC
-      pattern: "*.{yml,yaml}"
-      ontologies:
-        - edam: http://edamontology.org/format_3750 # YAML
-  - extra_multiqc_config:
-      type: file
-      description: Second optional config yml for MultiQC. Will override common sections
-        in multiqc_config.
-      pattern: "*.{yml,yaml}"
-      ontologies:
-        - edam: http://edamontology.org/format_3750 # YAML
-  - multiqc_logo:
-      type: file
-      description: Optional logo file for MultiQC
-      pattern: "*.{png}"
-      ontologies: []
-  - replace_names:
-      type: file
-      description: |
-        Optional two-column sample renaming file. First column a set of
-        patterns, second column a set of corresponding replacements. Passed via
-        MultiQC's `--replace-names` option.
-      pattern: "*.{tsv}"
-      ontologies:
-        - edam: http://edamontology.org/format_3475 # TSV
-  - sample_names:
-      type: file
-      description: |
-        Optional TSV file with headers, passed to the MultiQC --sample_names
-        argument.
-      pattern: "*.{tsv}"
-      ontologies:
-        - edam: http://edamontology.org/format_3475 # TSV
-output:
-  report:
-    - "*multiqc_report.html":
+  - - multiqc_files:
         type: file
-        description: MultiQC report file
-        pattern: "multiqc_report.html"
-        ontologies: []
-  data:
-    - "*_data":
-        type: directory
-        description: MultiQC data dir
-        pattern: "multiqc_data"
-  plots:
-    - "*_plots":
+        description: |
+          List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
+  - - multiqc_config:
         type: file
-        description: Plots created by MultiQC
-        pattern: "*_data"
-        ontologies: []
-  versions:
-    - versions.yml:
+        description: Optional config yml for MultiQC
+        pattern: "*.{yml,yaml}"
+  - - extra_multiqc_config:
         type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+        description: Second optional config yml for MultiQC. Will override common sections
+          in multiqc_config.
+        pattern: "*.{yml,yaml}"
+  - - multiqc_logo:
+        type: file
+        description: Optional logo file for MultiQC
+        pattern: "*.{png}"
+  - - replace_names:
+        type: file
+        description: |
+          Optional two-column sample renaming file. First column a set of
+          patterns, second column a set of corresponding replacements. Passed via
+          MultiQC's `--replace-names` option.
+        pattern: "*.{tsv}"
+  - - sample_names:
+        type: file
+        description: |
+          Optional TSV file with headers, passed to the MultiQC --sample_names
+          argument.
+        pattern: "*.{tsv}"
+output:
+  - report:
+      - "*multiqc_report.html":
+          type: file
+          description: MultiQC report file
+          pattern: "multiqc_report.html"
+  - data:
+      - "*_data":
+          type: directory
+          description: MultiQC data dir
+          pattern: "multiqc_data"
+  - plots:
+      - "*_plots":
+          type: file
+          description: Plots created by MultiQC
+          pattern: "*_data"
+  - versions:
+      - versions.yml:
+          type: file
+          description: File containing software versions
+          pattern: "versions.yml"
 authors:
   - "@abhi18av"
   - "@bunop"
diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap
index a88bafd..2fcbb5f 100644
--- a/modules/nf-core/multiqc/tests/main.nf.test.snap
+++ b/modules/nf-core/multiqc/tests/main.nf.test.snap
@@ -2,14 +2,14 @@
     "multiqc_versions_single": {
         "content": [
             [
-                "versions.yml:md5,737bb2c7cad54ffc2ec020791dc48b8f"
+                "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "24.10.4"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2025-10-27T13:33:24.356715"
+        "timestamp": "2024-10-02T17:51:46.317523"
     },
     "multiqc_stub": {
         "content": [
@@ -17,25 +17,25 @@
                 "multiqc_report.html",
                 "multiqc_data",
                 "multiqc_plots",
-                "versions.yml:md5,737bb2c7cad54ffc2ec020791dc48b8f"
+                "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "24.10.4"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2025-10-27T13:34:11.103619"
+        "timestamp": "2024-10-02T17:52:20.680978"
     },
     "multiqc_versions_config": {
         "content": [
             [
-                "versions.yml:md5,737bb2c7cad54ffc2ec020791dc48b8f"
+                "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916"
             ]
         ],
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "24.10.4"
+            "nf-test": "0.9.0",
+            "nextflow": "24.04.4"
         },
-        "timestamp": "2025-10-27T13:34:04.615233"
+        "timestamp": "2024-10-02T17:52:09.185842"
     }
 }
\ No newline at end of file
diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml
new file mode 100644
index 0000000..bea6c0d
--- /dev/null
+++ b/modules/nf-core/multiqc/tests/tags.yml
@@ -0,0 +1,2 @@
+multiqc:
+  - modules/nf-core/multiqc/**
diff --git a/nextflow.config b/nextflow.config
index 0975f17..98dcea4 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -32,15 +32,13 @@ params {
     email_on_fail                = null
     plaintext_email              = false
     monochrome_logs              = false
-    hook_url                     = System.getenv('HOOK_URL')
+    hook_url                     = null
     help                         = false
     help_full                    = false
     show_hidden                  = false
     version                      = false
     pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/'
-    trace_report_suffix          = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')
-
-    // Config options
+    trace_report_suffix          = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')// Config options
     config_profile_name        = null
     config_profile_description = null
 
@@ -93,18 +91,7 @@ profiles {
         apptainer.enabled       = false
         docker.runOptions       = '-u $(id -u):$(id -g)'
     }
-    arm64 {
-        process.arch            = 'arm64'
-        // TODO https://github.com/nf-core/modules/issues/6694
-        // For now if you're using arm64 you have to use wave for the sake of the maintainers
-        // wave profile
-        apptainer.ociAutoPull   = true
-        singularity.ociAutoPull = true
-        wave.enabled            = true
-        wave.freeze             = true
-        wave.strategy           = 'conda,container'
-    }
-    emulate_amd64 {
+    arm {
         docker.runOptions       = '-u $(id -u):$(id -g) --platform=linux/amd64'
     }
     singularity {
@@ -161,25 +148,28 @@ profiles {
         wave.freeze             = true
         wave.strategy           = 'conda,container'
     }
-    gpu {
-        docker.runOptions       = '-u $(id -u):$(id -g) --gpus all'
-        apptainer.runOptions    = '--nv'
-        singularity.runOptions  = '--nv'
+    gitpod {
+        executor.name           = 'local'
+        executor.cpus           = 4
+        executor.memory         = 8.GB
+        process {
+            resourceLimits = [
+                memory: 8.GB,
+                cpus  : 4,
+                time  : 1.h
+            ]
+        }
     }
     test      { includeConfig 'conf/test.config'      }
     test_full { includeConfig 'conf/test_full.config' }
 }
 
-// Load nf-core custom profiles from different institutions
-
-// If params.custom_config_base is set AND either the NXF_OFFLINE environment variable is not set or params.custom_config_base is a local path, the nfcore_custom.config file from the specified base path is included.
-// Load nf-core/deepmutscan custom profiles from different institutions.
-includeConfig params.custom_config_base && (!System.getenv('NXF_OFFLINE') || !params.custom_config_base.startsWith('http')) ? "${params.custom_config_base}/nfcore_custom.config" : "/dev/null"
-
+// Load nf-core custom profiles from different Institutions
+includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/nfcore_custom.config" : "/dev/null"
 
 // Load nf-core/deepmutscan custom profiles from different institutions.
 // TODO nf-core: Optionally, you can add a pipeline-specific nf-core config at https://github.com/nf-core/configs
-// includeConfig params.custom_config_base && (!System.getenv('NXF_OFFLINE') || !params.custom_config_base.startsWith('http')) ? "${params.custom_config_base}/pipeline/deepmutscan.config" : "/dev/null"
+// includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/deepmutscan.config" : "/dev/null"
 
 // Set default registry for Apptainer, Docker, Podman, Charliecloud and Singularity independent of -profile
 // Will not be used unless Apptainer / Docker / Podman / Charliecloud / Singularity are enabled
@@ -205,14 +195,14 @@ env {
 }
 
 // Set bash options
-process.shell = [
-    "bash",
-    "-C",         // No clobber - prevent output redirection from overwriting files.
-    "-e",         // Exit if a tool returns a non-zero status/exit code
-    "-u",         // Treat unset variables and parameters as an error
-    "-o",         // Returns the status of the last command to exit..
-    "pipefail"    //   ..with a non-zero status or zero if all successfully execute
-]
+process.shell = """\
+bash
+
+set -e # Exit if a tool returns a non-zero status/exit code
+set -u # Treat unset variables and parameters as an error
+set -o pipefail # Returns the status of the last command to exit with a non-zero status or zero if all successfully execute
+set -C # No clobber - prevent output redirection from overwriting files.
+"""
 
 // Disable process selector warnings by default. Use debug profile to enable warnings.
 nextflow.enable.configProcessNamesValidation = false
@@ -236,6 +226,7 @@ dag {
 
 manifest {
     name            = 'nf-core/deepmutscan'
+    author          = """Benjamin Wehnert & Max Stammnitz""" // The author field is deprecated from Nextflow version 24.10.0, use contributors instead
     contributors    = [
         // TODO nf-core: Update the field with the details of the contributors to your pipeline. New with Nextflow version 24.10.0
         [
@@ -251,19 +242,46 @@ manifest {
     description     = """Until now, most Deep Mutational Scanning (DMS) experiments relied on variant-specific barcoded libraries for sequencing. This method enabled DMS on large proteins and led to many great publications. Recently, efforts have increased to make use of the classic and more simple random fragmentation-based short-read sequencing (“shotgun-sequencing”). This saves time and money and due to its simpler experimental design is less prone to mistakes. dmscore handles the essential computational steps, processing the raw FASTQ files and generating a count table of variants. Along the way, it provides multiple QC metrics, enabling users to quickly evaluate the success of their experimental setup."""
     mainScript      = 'main.nf'
     defaultBranch   = 'master'
-    nextflowVersion = '!>=25.04.0'
+    nextflowVersion = '!>=24.04.2'
     version         = '1.0.0'
     doi             = ''
 }
 
 // Nextflow plugins
 plugins {
-    id 'nf-schema@2.5.1' // Validation of pipeline parameters and creation of an input channel from a sample sheet
+    id 'nf-schema@2.3.0' // Validation of pipeline parameters and creation of an input channel from a sample sheet
 }
 
 validation {
     defaultIgnoreParams = ["genomes"]
     monochromeLogs = params.monochrome_logs
+    help {
+        enabled = true
+        command = "nextflow run nf-core/deepmutscan -profile <docker/singularity/.../institute> --input samplesheet.csv --outdir <OUTDIR>"
+        fullParameter = "help_full"
+        showHiddenParameter = "show_hidden"
+        beforeText = """
+-\033[2m----------------------------------------------------\033[0m-
+                                        \033[0;32m,--.\033[0;30m/\033[0;32m,-.\033[0m
+\033[0;34m        ___     __   __   __   ___     \033[0;32m/,-._.--~\'\033[0m
+\033[0;34m  |\\ | |__  __ /  ` /  \\ |__) |__         \033[0;33m}  {\033[0m
+\033[0;34m  | \\| |       \\__, \\__/ |  \\ |___     \033[0;32m\\`-._,-`-,\033[0m
+                                        \033[0;32m`._,._,\'\033[0m
+\033[0;35m  nf-core/deepmutscan ${manifest.version}\033[0m
+-\033[2m----------------------------------------------------\033[0m-
+"""
+        afterText = """${manifest.doi ? "\n* The pipeline\n" : ""}${manifest.doi.tokenize(",").collect { "    https://doi.org/${it.trim().replace('https://doi.org/','')}"}.join("\n")}${manifest.doi ? "\n" : ""}
+* The nf-core framework
+    https://doi.org/10.1038/s41587-020-0439-x
+
+* Software dependencies
+    https://github.com/nf-core/deepmutscan/blob/master/CITATIONS.md
+"""
+    }
+    summary {
+        beforeText = validation.help.beforeText
+        afterText = validation.help.afterText
+    }
 }
 
 // Load modules.config for DSL2 module specific options
diff --git a/nextflow_schema.json b/nextflow_schema.json
index c232fc5..735acee 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -224,18 +224,6 @@
                     "fa_icon": "far calendar",
                     "description": "Suffix to add to the trace report filename. Default is the date and time in the format yyyy-MM-dd_HH-mm-ss.",
                     "hidden": true
-                },
-                "help": {
-                    "type": ["boolean", "string"],
-                    "description": "Display the help message."
-                },
-                "help_full": {
-                    "type": "boolean",
-                    "description": "Display the full detailed help message."
-                },
-                "show_hidden": {
-                    "type": "boolean",
-                    "description": "Display hidden parameters in the help message (only works when --help or --help_full are provided)."
                 }
             }
         }
diff --git a/nf-test.config b/nf-test.config
deleted file mode 100644
index 3a1fff5..0000000
--- a/nf-test.config
+++ /dev/null
@@ -1,24 +0,0 @@
-config {
-    // location for all nf-test tests
-    testsDir "."
-
-    // nf-test directory including temporary files for each test
-    workDir System.getenv("NFT_WORKDIR") ?: ".nf-test"
-
-    // location of an optional nextflow.config file specific for executing tests
-    configFile "tests/nextflow.config"
-
-    // ignore tests coming from the nf-core/modules repo
-    ignore 'modules/nf-core/**/tests/*', 'subworkflows/nf-core/**/tests/*'
-
-    // run all test with defined profile(s) from the main nextflow.config
-    profile "test"
-
-    // list of filenames or patterns that should be trigger a full test run
-    triggers 'nextflow.config', 'nf-test.config', 'conf/test.config', 'tests/nextflow.config', 'tests/.nftignore'
-
-    // load the necessary plugins
-    plugins {
-        load "nft-utils@0.0.3"
-    }
-}
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index 57aaf4e..a21ab42 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -22,8 +22,8 @@
             "@id": "./",
             "@type": "Dataset",
             "creativeWorkStatus": "Stable",
-            "datePublished": "2025-11-20T09:32:07+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-deepmutscan_logo_dark.png\">\n    <img alt=\"nf-core/deepmutscan\" src=\"docs/images/nf-core-deepmutscan_logo_light.png\">\n  </picture>\n</h1>\n\n[![Open in GitHub Codespaces](https://img.shields.io/badge/Open_In_GitHub_Codespaces-black?labelColor=grey&logo=github)](https://github.com/codespaces/new/nf-core/deepmutscan)\n[![GitHub Actions CI Status](https://github.com/nf-core/deepmutscan/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/deepmutscan/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/deepmutscan/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/deepmutscan/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/deepmutscan/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A525.04.0-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.5.1-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.5.1)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/deepmutscan)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23deepmutscan-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/deepmutscan)[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/deepmutscan** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/guidelines/graphic_design/workflow_diagrams#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/deepmutscan \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/deepmutscan/usage) and the [parameter documentation](https://nf-co.re/deepmutscan/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/deepmutscan/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/deepmutscan/output).\n\n## Credits\n\nnf-core/deepmutscan was originally written by Benjamin Wehnert & Max Stammnitz.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#deepmutscan` channel](https://nfcore.slack.com/channels/deepmutscan) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/deepmutscan for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->\n\n<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
+            "datePublished": "2026-02-07T16:49:52+00:00",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-deepmutscan_logo_dark.png\">\n    <img alt=\"nf-core/deepmutscan\" src=\"docs/images/nf-core-deepmutscan_logo_light.png\">\n  </picture>\n</h1>\n\n[![GitHub Actions CI Status](https://github.com/nf-core/deepmutscan/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/deepmutscan/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/deepmutscan/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/deepmutscan/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/deepmutscan/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/deepmutscan)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23deepmutscan-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/deepmutscan)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/deepmutscan** is a bioinformatics pipeline that ...\n\n<!-- TODO nf-core:\n   Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the\n   major pipeline sections and the types of output it produces. You're giving an overview to someone new\n   to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction\n-->\n\n<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core\n     workflows use the \"tube map\" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples.   -->\n<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.\n     Explain what rows and columns represent. For instance (please edit as appropriate):\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nsample,fastq_1,fastq_2\nCONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a fastq file (single-end) or a pair of fastq files (paired end).\n\n-->\n\nNow, you can run the pipeline using:\n\n<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->\n\n```bash\nnextflow run nf-core/deepmutscan \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/deepmutscan/usage) and the [parameter documentation](https://nf-co.re/deepmutscan/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/deepmutscan/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/deepmutscan/output).\n\n## Credits\n\nnf-core/deepmutscan was originally written by Benjamin Wehnert & Max Stammnitz.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n<!-- TODO nf-core: If applicable, make list of people who have also contributed -->\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#deepmutscan` channel](https://nfcore.slack.com/channels/deepmutscan) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->\n<!-- If you use nf-core/deepmutscan for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->\n\n<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n",
             "hasPart": [
                 {
                     "@id": "main.nf"
@@ -99,7 +99,7 @@
             },
             "mentions": [
                 {
-                    "@id": "#280e9a69-af96-4e4c-80bc-ae5ef9dda531"
+                    "@id": "#8858aaed-f60a-4dec-b22f-e46658409de2"
                 }
             ],
             "name": "nf-core/deepmutscan"
@@ -121,9 +121,13 @@
         },
         {
             "@id": "main.nf",
-            "@type": ["File", "SoftwareSourceCode", "ComputationalWorkflow"],
+            "@type": [
+                "File",
+                "SoftwareSourceCode",
+                "ComputationalWorkflow"
+            ],
             "dateCreated": "",
-            "dateModified": "2025-11-20T09:32:07Z",
+            "dateModified": "2026-02-07T17:49:52Z",
             "dct:conformsTo": "https://bioschemas.org/profiles/ComputationalWorkflow/1.0-RELEASE/",
             "keywords": [
                 "nf-core",
@@ -133,16 +137,25 @@
                 "genotype-phenotype",
                 "shotgun-sequencing"
             ],
-            "license": ["MIT"],
-            "name": ["nf-core/deepmutscan"],
+            "license": [
+                "MIT"
+            ],
+            "name": [
+                "nf-core/deepmutscan"
+            ],
             "programmingLanguage": {
                 "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow"
             },
             "sdPublisher": {
                 "@id": "https://nf-co.re/"
             },
-            "url": ["https://github.com/nf-core/deepmutscan", "https://nf-co.re/deepmutscan/1.0.0/"],
-            "version": ["1.0.0"]
+            "url": [
+                "https://github.com/nf-core/deepmutscan",
+                "https://nf-co.re/deepmutscan/1.0.0/"
+            ],
+            "version": [
+                "1.0.0"
+            ]
         },
         {
             "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow",
@@ -154,14 +167,14 @@
             "url": {
                 "@id": "https://www.nextflow.io/"
             },
-            "version": "!>=25.04.0"
+            "version": "!>=24.04.2"
         },
         {
-            "@id": "#280e9a69-af96-4e4c-80bc-ae5ef9dda531",
+            "@id": "#8858aaed-f60a-4dec-b22f-e46658409de2",
             "@type": "TestSuite",
             "instance": [
                 {
-                    "@id": "#ac596659-2e2a-421a-b5d0-69df6db85229"
+                    "@id": "#3e21805b-327c-46c1-9973-e41f49b26535"
                 }
             ],
             "mainEntity": {
@@ -170,10 +183,10 @@
             "name": "Test suite for nf-core/deepmutscan"
         },
         {
-            "@id": "#ac596659-2e2a-421a-b5d0-69df6db85229",
+            "@id": "#3e21805b-327c-46c1-9973-e41f49b26535",
             "@type": "TestInstance",
             "name": "GitHub Actions workflow for testing nf-core/deepmutscan",
-            "resource": "repos/nf-core/deepmutscan/actions/workflows/nf-test.yml",
+            "resource": "repos/nf-core/deepmutscan/actions/workflows/ci.yml",
             "runsOn": {
                 "@id": "https://w3id.org/ro/terms/test#GithubService"
             },
@@ -299,4 +312,4 @@
             "url": "https://nf-co.re/"
         }
     ]
-}
+}
\ No newline at end of file
diff --git a/subworkflows/local/utils_nfcore_deepmutscan_pipeline/main.nf b/subworkflows/local/utils_nfcore_deepmutscan_pipeline/main.nf
index 3b2c26a..cb2380d 100644
--- a/subworkflows/local/utils_nfcore_deepmutscan_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_deepmutscan_pipeline/main.nf
@@ -11,7 +11,6 @@
 include { UTILS_NFSCHEMA_PLUGIN     } from '../../nf-core/utils_nfschema_plugin'
 include { paramsSummaryMap          } from 'plugin/nf-schema'
 include { samplesheetToList         } from 'plugin/nf-schema'
-include { paramsHelp                } from 'plugin/nf-schema'
 include { completionEmail           } from '../../nf-core/utils_nfcore_pipeline'
 include { completionSummary         } from '../../nf-core/utils_nfcore_pipeline'
 include { imNotification            } from '../../nf-core/utils_nfcore_pipeline'
@@ -33,13 +32,10 @@ workflow PIPELINE_INITIALISATION {
     nextflow_cli_args //   array: List of positional nextflow CLI args
     outdir            //  string: The output directory where the results will be saved
     input             //  string: Path to input samplesheet
-    help              // boolean: Display help message and exit
-    help_full         // boolean: Show the full help message
-    show_hidden       // boolean: Show hidden parameters in the help message
 
     main:
 
-    ch_versions = channel.empty()
+    ch_versions = Channel.empty()
 
     //
     // Print version and exit if required and dump pipeline parameters to JSON file
@@ -54,35 +50,10 @@ workflow PIPELINE_INITIALISATION {
     //
     // Validate parameters and generate parameter summary to stdout
     //
-    before_text = """
--\033[2m----------------------------------------------------\033[0m-
-                                        \033[0;32m,--.\033[0;30m/\033[0;32m,-.\033[0m
-\033[0;34m        ___     __   __   __   ___     \033[0;32m/,-._.--~\'\033[0m
-\033[0;34m  |\\ | |__  __ /  ` /  \\ |__) |__         \033[0;33m}  {\033[0m
-\033[0;34m  | \\| |       \\__, \\__/ |  \\ |___     \033[0;32m\\`-._,-`-,\033[0m
-                                        \033[0;32m`._,._,\'\033[0m
-\033[0;35m  nf-core/deepmutscan ${workflow.manifest.version}\033[0m
--\033[2m----------------------------------------------------\033[0m-
-"""
-    after_text = """${workflow.manifest.doi ? "\n* The pipeline\n" : ""}${workflow.manifest.doi.tokenize(",").collect { doi -> "    https://doi.org/${doi.trim().replace('https://doi.org/','')}"}.join("\n")}${workflow.manifest.doi ? "\n" : ""}
-* The nf-core framework
-    https://doi.org/10.1038/s41587-020-0439-x
-
-* Software dependencies
-    https://github.com/nf-core/deepmutscan/blob/master/CITATIONS.md
-"""
-    command = "nextflow run ${workflow.manifest.name} -profile <docker/singularity/.../institute> --input samplesheet.csv --outdir <OUTDIR>"
-
     UTILS_NFSCHEMA_PLUGIN (
         workflow,
         validate_params,
-        null,
-        help,
-        help_full,
-        show_hidden,
-        before_text,
-        after_text,
-        command
+        null
     )
 
     //
@@ -101,7 +72,7 @@ workflow PIPELINE_INITIALISATION {
     // Create channel from input file provided through params.input
     //
 
-    channel
+    Channel
         .fromList(samplesheetToList(params.input, "${projectDir}/assets/schema_input.json"))
         .map {
             meta, fastq_1, fastq_2 ->
@@ -290,3 +261,4 @@ def methodsDescriptionText(mqc_methods_yaml) {
 
     return description_html.toString()
 }
+
diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml
new file mode 100644
index 0000000..f847611
--- /dev/null
+++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml
@@ -0,0 +1,2 @@
+subworkflows/utils_nextflow_pipeline:
+  - subworkflows/nf-core/utils_nextflow_pipeline/**
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
index 2f30e9a..bfd2587 100644
--- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf
@@ -98,7 +98,7 @@ def workflowVersionToYAML() {
 // Get channel of software versions used in pipeline in YAML format
 //
 def softwareVersionsToYAML(ch_versions) {
-    return ch_versions.unique().map { version -> processVersionsFromYAML(version) }.unique().mix(channel.of(workflowVersionToYAML()))
+    return ch_versions.unique().map { version -> processVersionsFromYAML(version) }.unique().mix(Channel.of(workflowVersionToYAML()))
 }
 
 //
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml
new file mode 100644
index 0000000..ac8523c
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml
@@ -0,0 +1,2 @@
+subworkflows/utils_nfcore_pipeline:
+  - subworkflows/nf-core/utils_nfcore_pipeline/**
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/main.nf b/subworkflows/nf-core/utils_nfschema_plugin/main.nf
index ee4738c..4994303 100644
--- a/subworkflows/nf-core/utils_nfschema_plugin/main.nf
+++ b/subworkflows/nf-core/utils_nfschema_plugin/main.nf
@@ -4,7 +4,6 @@
 
 include { paramsSummaryLog   } from 'plugin/nf-schema'
 include { validateParameters } from 'plugin/nf-schema'
-include { paramsHelp         } from 'plugin/nf-schema'
 
 workflow UTILS_NFSCHEMA_PLUGIN {
 
@@ -16,56 +15,29 @@ workflow UTILS_NFSCHEMA_PLUGIN {
                         //           when this input is empty it will automatically use the configured schema or
                         //           "${projectDir}/nextflow_schema.json" as default. This input should not be empty
                         //           for meta pipelines
-    help                // boolean:  show help message
-    help_full           // boolean:  show full help message
-    show_hidden         // boolean:  show hidden parameters in help message
-    before_text         // string:   text to show before the help message and parameters summary
-    after_text          // string:   text to show after the help message and parameters summary
-    command             // string:   an example command of the pipeline
 
     main:
 
-    if(help || help_full) {
-        help_options = [
-            beforeText: before_text,
-            afterText: after_text,
-            command: command,
-            showHidden: show_hidden,
-            fullHelp: help_full,
-        ]
-        if(parameters_schema) {
-            help_options << [parametersSchema: parameters_schema]
-        }
-        log.info paramsHelp(
-            help_options,
-            params.help instanceof String ? params.help : "",
-        )
-        exit 0
-    }
-
     //
     // Print parameter summary to stdout. This will display the parameters
     // that differ from the default given in the JSON schema
     //
-
-    summary_options = [:]
     if(parameters_schema) {
-        summary_options << [parametersSchema: parameters_schema]
+        log.info paramsSummaryLog(input_workflow, parameters_schema:parameters_schema)
+    } else {
+        log.info paramsSummaryLog(input_workflow)
     }
-    log.info before_text
-    log.info paramsSummaryLog(summary_options, input_workflow)
-    log.info after_text
 
     //
     // Validate the parameters using nextflow_schema.json or the schema
     // given via the validation.parametersSchema configuration option
     //
     if(validate_params) {
-        validateOptions = [:]
         if(parameters_schema) {
-            validateOptions << [parametersSchema: parameters_schema]
+            validateParameters(parameters_schema:parameters_schema)
+        } else {
+            validateParameters()
         }
-        validateParameters(validateOptions)
     }
 
     emit:
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test
index c977917..8fb3016 100644
--- a/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test
+++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test
@@ -25,12 +25,6 @@ nextflow_workflow {
                 input[0] = workflow
                 input[1] = validate_params
                 input[2] = ""
-                input[3] = false
-                input[4] = false
-                input[5] = false
-                input[6] = ""
-                input[7] = ""
-                input[8] = ""
                 """
             }
         }
@@ -57,12 +51,6 @@ nextflow_workflow {
                 input[0] = workflow
                 input[1] = validate_params
                 input[2] = ""
-                input[3] = false
-                input[4] = false
-                input[5] = false
-                input[6] = ""
-                input[7] = ""
-                input[8] = ""
                 """
             }
         }
@@ -89,12 +77,6 @@ nextflow_workflow {
                 input[0] = workflow
                 input[1] = validate_params
                 input[2] = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json"
-                input[3] = false
-                input[4] = false
-                input[5] = false
-                input[6] = ""
-                input[7] = ""
-                input[8] = ""
                 """
             }
         }
@@ -121,12 +103,6 @@ nextflow_workflow {
                 input[0] = workflow
                 input[1] = validate_params
                 input[2] = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json"
-                input[3] = false
-                input[4] = false
-                input[5] = false
-                input[6] = ""
-                input[7] = ""
-                input[8] = ""
                 """
             }
         }
@@ -138,36 +114,4 @@ nextflow_workflow {
             )
         }
     }
-
-    test("Should create a help message") {
-
-        when {
-
-            params {
-                test_data   = ''
-                outdir      = null
-            }
-
-            workflow {
-                """
-                validate_params = true
-                input[0] = workflow
-                input[1] = validate_params
-                input[2] = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json"
-                input[3] = true
-                input[4] = false
-                input[5] = false
-                input[6] = "Before"
-                input[7] = "After"
-                input[8] = "nextflow run test/test"
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success }
-            )
-        }
-    }
 }
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config
index 8d8c737..0907ac5 100644
--- a/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config
+++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config
@@ -1,8 +1,8 @@
 plugins {
-    id "nf-schema@2.5.1"
+    id "nf-schema@2.1.0"
 }
 
 validation {
     parametersSchema = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json"
     monochromeLogs = true
-}
+}
\ No newline at end of file
diff --git a/tests/.nftignore b/tests/.nftignore
deleted file mode 100644
index e128a12..0000000
--- a/tests/.nftignore
+++ /dev/null
@@ -1,12 +0,0 @@
-.DS_Store
-multiqc/multiqc_data/fastqc_top_overrepresented_sequences_table.txt
-multiqc/multiqc_data/multiqc.parquet
-multiqc/multiqc_data/multiqc.log
-multiqc/multiqc_data/multiqc_data.json
-multiqc/multiqc_data/multiqc_sources.txt
-multiqc/multiqc_data/multiqc_software_versions.txt
-multiqc/multiqc_data/llms-full.txt
-multiqc/multiqc_plots/{svg,pdf,png}/*.{svg,pdf,png}
-multiqc/multiqc_report.html
-fastqc/*_fastqc.{html,zip}
-pipeline_info/*.{html,json,txt,yml}
diff --git a/tests/default.nf.test b/tests/default.nf.test
deleted file mode 100644
index efb3834..0000000
--- a/tests/default.nf.test
+++ /dev/null
@@ -1,33 +0,0 @@
-nextflow_pipeline {
-
-    name "Test pipeline"
-    script "../main.nf"
-    tag "pipeline"
-
-    test("-profile test") {
-
-        when {
-            params {
-                outdir = "$outputDir"
-            }
-        }
-
-        then {
-            // stable_name: All files + folders in ${params.outdir}/ with a stable name
-            def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
-            // stable_path: All files in ${params.outdir}/ with stable content
-            def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore')
-            assertAll(
-                { assert workflow.success},
-                { assert snapshot(
-                    // pipeline versions.yml file for multiqc from which Nextflow version is removed because we test pipelines on multiple Nextflow versions
-                    removeNextflowVersion("$outputDir/pipeline_info/nf_core_deepmutscan_software_mqc_versions.yml"),
-                    // All stable path name, with a relative path
-                    stable_name,
-                    // All files with stable contents
-                    stable_path
-                ).match() }
-            )
-        }
-    }
-}
diff --git a/tests/nextflow.config b/tests/nextflow.config
deleted file mode 100644
index 662ccd0..0000000
--- a/tests/nextflow.config
+++ /dev/null
@@ -1,14 +0,0 @@
-/*
-========================================================================================
-    Nextflow config file for running nf-test tests
-========================================================================================
-*/
-
-// TODO nf-core: Specify any additional parameters here
-// Or any resources requirements
-params {
-    modules_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/'
-    pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/deepmutscan'
-}
-
-aws.client.anonymous = true // fixes S3 access issues on self-hosted runners
diff --git a/workflows/deepmutscan.nf b/workflows/deepmutscan.nf
index 12ac5d0..2b6bf8d 100644
--- a/workflows/deepmutscan.nf
+++ b/workflows/deepmutscan.nf
@@ -22,8 +22,8 @@ workflow DEEPMUTSCAN {
     ch_samplesheet // channel: samplesheet read in from --input
     main:
 
-    ch_versions = channel.empty()
-    ch_multiqc_files = channel.empty()
+    ch_versions = Channel.empty()
+    ch_multiqc_files = Channel.empty()
     //
     // MODULE: Run FastQC
     //
@@ -36,25 +36,7 @@ workflow DEEPMUTSCAN {
     //
     // Collate and save software versions
     //
-    def topic_versions = Channel.topic("versions")
-        .distinct()
-        .branch { entry ->
-            versions_file: entry instanceof Path
-            versions_tuple: true
-        }
-
-    def topic_versions_string = topic_versions.versions_tuple
-        .map { process, tool, version ->
-            [ process[process.lastIndexOf(':')+1..-1], "  ${tool}: ${version}" ]
-        }
-        .groupTuple(by:0)
-        .map { process, tool_versions ->
-            tool_versions.unique().sort()
-            "${process}:\n${tool_versions.join('\n')}"
-        }
-
-    softwareVersionsToYAML(ch_versions.mix(topic_versions.versions_file))
-        .mix(topic_versions_string)
+    softwareVersionsToYAML(ch_versions)
         .collectFile(
             storeDir: "${params.outdir}/pipeline_info",
             name: 'nf_core_'  +  'deepmutscan_software_'  + 'mqc_'  + 'versions.yml',
@@ -66,24 +48,24 @@ workflow DEEPMUTSCAN {
     //
     // MODULE: MultiQC
     //
-    ch_multiqc_config        = channel.fromPath(
+    ch_multiqc_config        = Channel.fromPath(
         "$projectDir/assets/multiqc_config.yml", checkIfExists: true)
     ch_multiqc_custom_config = params.multiqc_config ?
-        channel.fromPath(params.multiqc_config, checkIfExists: true) :
-        channel.empty()
+        Channel.fromPath(params.multiqc_config, checkIfExists: true) :
+        Channel.empty()
     ch_multiqc_logo          = params.multiqc_logo ?
-        channel.fromPath(params.multiqc_logo, checkIfExists: true) :
-        channel.empty()
+        Channel.fromPath(params.multiqc_logo, checkIfExists: true) :
+        Channel.empty()
 
     summary_params      = paramsSummaryMap(
         workflow, parameters_schema: "nextflow_schema.json")
-    ch_workflow_summary = channel.value(paramsSummaryMultiqc(summary_params))
+    ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params))
     ch_multiqc_files = ch_multiqc_files.mix(
         ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
     ch_multiqc_custom_methods_description = params.multiqc_methods_description ?
         file(params.multiqc_methods_description, checkIfExists: true) :
         file("$projectDir/assets/methods_description_template.yml", checkIfExists: true)
-    ch_methods_description                = channel.value(
+    ch_methods_description                = Channel.value(
         methodsDescriptionText(ch_multiqc_custom_methods_description))
 
     ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions)

From 2fa2a24ce7959f6b3137d9ce098f4075927ef0e4 Mon Sep 17 00:00:00 2001
From: BenjaminWehnert1008
 <84199433+BenjaminWehnert1008@users.noreply.github.com>
Date: Mon, 9 Feb 2026 11:55:42 +0100
Subject: [PATCH 3/4] Update .nf-core.yml
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

Co-authored-by: Matthias Hörtenhuber <mashehu@users.noreply.github.com>
---
 .nf-core.yml | 2 +-
 1 file changed, 1 insertion(+), 1 deletion(-)

diff --git a/.nf-core.yml b/.nf-core.yml
index f23f386..d2ae8e1 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -1,5 +1,5 @@
 lint: {}
-nf_core_version: 3.1.2
+nf_core_version: 3.5.2
 repository_type: pipeline
 template:
   author: Benjamin Wehnert & Max Stammnitz

From 2ddc12a352e8a1e85302014e5676b4243eb14098 Mon Sep 17 00:00:00 2001
From: nf-core-bot <core@nf-co.re>
Date: Mon, 9 Feb 2026 10:58:55 +0000
Subject: [PATCH 4/4] [automated] Fix code linting

---
 docs/output.md       | 18 ++++++++++--------
 docs/usage.md        |  1 +
 nextflow_schema.json |  4 ++--
 3 files changed, 13 insertions(+), 10 deletions(-)

diff --git a/docs/output.md b/docs/output.md
index 116e114..dfbbbef 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -75,23 +75,24 @@ This directory is created during the second series of steps of the pipeline, fea
 <summary>Output files</summary>
 
 - `library_QC/`
+
   - `counts_heatmap.pdf`: a complete heatmap of absolute mutant counts, stratified by mutant amino acid (Y-axis) per position (X-axis)
-![Count heatmap](images/library_QC_counts_heatmap.png)
+    ![Count heatmap](images/library_QC_counts_heatmap.png)
 
   - `counts_per_cov_heatmap.pdf`: as above, but as a fraction of the total sequencing coverage
   - `logdiff_plot.pdf`: sorted, log-scale coverage distribution of all mutants
-![Logarithmic count differences](images/library_QC_logdiff_plot.png)
+    ![Logarithmic count differences](images/library_QC_logdiff_plot.png)
 
   - `logdiff_varying_bases.pdf`: as above, but stratified by hamming distance to the wildtype nucleotide sequence (colour shading)
   - `rolling_coverage.pdf`: sliding-window rolling coverage
-![Rolling coverage](images/library_QC_rolling_coverage.png)
+    ![Rolling coverage](images/library_QC_rolling_coverage.png)
 
   - `rolling_counts.pdf`: sliding-window rolling coverage, stratified by hamming distance to the wildtype nucleotide sequence (colour shading)
-![Rolling counts](images/library_QC_rolling_counts.png)
+    ![Rolling counts](images/library_QC_rolling_counts.png)
 
   - `rolling_counts_per_cov.pdf`: as above, but as a fraction of the total sequencing coverage
   - `SeqDepth.pdf` (optional via the `--run_seqdepth` argument): rarefaction curve of the sequencing coverage and how it relates to the percentage of programmed variants detected
-![Sequencing coverage rarefaction](images/library_QC_SeqDepth.png)
+    ![Sequencing coverage rarefaction](images/library_QC_SeqDepth.png)
 
 </details>
 
@@ -103,15 +104,16 @@ This directory is created during the final series of steps of the pipeline, feat
 <summary>Output files</summary>
 
 - `fitness/`
+
   - `counts_merged.tsv`: summarised gene variant counts across all input and output samples
   - `default_results/fitness_estimation_count_correlation.pdf`: pair-wise replicate variant count scatterplots and correlations between all specified samples
-![Variant count correlation(s)](images/fitness_estimation_count_correlation.png)
+    ![Variant count correlation(s)](images/fitness_estimation_count_correlation.png)
 
   - `default_results/fitness_estimation_fitness_correlation.pdf`: pair-wise fitness replicate scatterplots and correlations between all specified output samples
-![Fitness correlation(s)](images/fitness_estimation_fitness_correlation.png)
+    ![Fitness correlation(s)](images/fitness_estimation_fitness_correlation.png)
 
   - `default_results/fitness_heatmap.pdf`: a complete heatmap of absolute mutant counts, stratified by mutant amino acid (Y-axis) per position (X-axis)
-![Default fitness heatmap](images/fitness_heatmap.png)
+    ![Default fitness heatmap](images/fitness_heatmap.png)
 
   - `default_results/fitness_estimation.tsv`: table file with all fitness and fitness error estimates calculated
   - `DiMSum_results/dimsum_results/` (optional): subfolder with the full set of [DiMSum](https://github.com/lehner-lab/DiMSum) outputs, including the associated `.HTML` report, `.Rdata` and `.tsv` files with fitness and fitness error estimates
diff --git a/docs/usage.md b/docs/usage.md
index 9202adb..c4067f6 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -153,6 +153,7 @@ This module will feature strategies to distinguish true single nucleotide varian
 The final step of the pipeline will perform fitness estimation based on mutation counts. By default, we calculate fitness scores as the logarithm of variants' output to input ratio, normalised to that of the provided wildtype nucleotide sequence.
 
 Future expansions may include:
+
 - Integration of other popular fitness inference tools, including [DiMSum](https://github.com/lehner-lab/DiMSum), [Enrich2](https://github.com/FowlerLab/Enrich2), [rosace](https://github.com/pimentellab/rosace/) and [mutscan](https://github.com/fmicompbio/mutscan)
 - Standardised output formats for downstream analyses and comparison
 
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 67b684f..706dcb2 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -52,12 +52,12 @@
                     "minimum": 1,
                     "default": 3
                 },
-		"sliding_window_size": {
+                "sliding_window_size": {
                     "type": "integer",
                     "description": "To flatten graphs in plots (e.g. `GLOBAL_POS_BIASES_COUNTS` function)",
                     "default": 10
                 },
-		"aimed_cov": {
+                "aimed_cov": {
                     "type": "integer",
                     "description": "aimed coverage (assuming equal spread) to visualize threshold in plots",
                     "default": 100