diff --git a/CHANGELOG.md b/CHANGELOG.md
index 00339c8ff..45432e96c 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -56,6 +56,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - Fixed bug in `GFFREAD` that caused output `gffread_fasta` not being produced [#565](https://github.com/nf-core/rnafusion/issues/565)
 - Fixed bug in `FUSIONCATCHER_DOWNLOAD` that caused an error when running with singularity profile [#573](https://github.com/nf-core/rnafusion/issues/573)
 - Fixed missing script `gtf2bed` which caused local module `GET_RRNA_TRANSCRIPTS` to fail [#602](https://github.com/nf-core/rnafusion/issues/602)
+- Fixed samplesheet validation which caused errors on single-end reads [#612](https://github.com/nf-core/rnafusion/issues/612)
 
 ### Removed
 
diff --git a/assets/schema_input.json b/assets/schema_input.json
index a55008106..15ec66a85 100644
--- a/assets/schema_input.json
+++ b/assets/schema_input.json
@@ -33,6 +33,6 @@
                 "errorMessage": "Strandedness has to be forward, reverse, unstranded or unknown"
             }
         },
-        "required": ["sample", "fastq_1", "fastq_2", "strandedness"]
+        "required": ["sample", "fastq_1", "strandedness"]
     }
 }