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Hello, nh13, I have some question:
- I wonder if the mutations in the vcf file in the output of DWGSIM are randomly generated and how to control the counts of mutations.
- In the vcf file, what about the "AF" and "pl" int the "info“ field mean? If the AF=0.5, does that mean if I simulate 1,000,000 reads pairs at one time and 500,000 reads have the mutation?
Can you tell me about these two question? Great thanks!
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