Creating parameter specific (Arscin) transformation values

[SforR]

Hi everyone,

With my (flowcytometry) data I see that some channels have an optimal Arcsin transformation
that differs from the others. So I actually want to use different transformCofactors.
Has some one experience with that?
I have tried using a code transformCofactor = c(150,500,1000....) but that doesn't seem to do the trick.

Best,

Sam

[SamGG]

Hi,
The main point I noticed very recently is that citrus does not manage compensation at all (@rbruggner correct me if I am wrong). Because it was designed for mass cytometry. So the FCS have to be compensated first.
I will estimate how much work your request requires.
Best.

[SforR]

Hi Sam,

I didn't know that actually, luckily I always first apply compensation on my FCS data together with some pre-gating.
But thanks in adavance for looking and estimating the amount of work.

Cheers.