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Fixed Alignment issues#3

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rvanroey wants to merge 1 commit intoDSchreyer:devfrom
rvanroey:patch-1
Open

Fixed Alignment issues#3
rvanroey wants to merge 1 commit intoDSchreyer:devfrom
rvanroey:patch-1

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@rvanroey
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@rvanroey rvanroey commented May 22, 2021

The reason that pinaplpy does not find any successful reads is because it prioritises the .sam files over .bam files.
I've changed the code a bit to fix the issue temporarily (very sloppy code) and indexed the .bam file with Pysam.

PR checklist

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
  • If you've added a new tool - add to the software_versions process and a regex to scrape_software_versions.py
  • If you've added a new tool - have you followed the pipeline conventions in the contribution docs
  • If necessary, also make a PR on the nf-core/crisprquant branch on the nf-core/test-datasets repository.
  • Make sure your code lints (nf-core lint .).
  • Ensure the test suite passes (nextflow run . -profile test,docker).
  • Usage Documentation in docs/usage.md is updated.
  • Output Documentation in docs/output.md is updated.
  • CHANGELOG.md is updated.
  • README.md is updated (including new tool citations and authors/contributors).

The reason that pinaplpy does not find any successful reads is because it prioritises the .sam files over .bam files.
I've changed the code a bit to fix the issue temporarily (very sloppy code) and indexed the .bam file with Pysam.



Currently, pinaplpy does not find any successful reads, however the bam files do look ok
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