Monocle is an analysis toolkit for single-cell RNA-Seq experiments. To use this package, you will need the R statistical computing environment (version 3.0 or later) and several packages available through Bioconductor and CRAN.
We highly recommend installation of Monocle through the bioconductor project. See here for details:
https://bioconductor.org/packages/release/bioc/html/monocle.html
News and announcements for the Monocle project will appear here:
http://cole-trapnell-lab.github.io/monocle-release/
This release supports Mac OS X and Linux. Windows may support this build, but Monocle has not been tested on Windows yet.