This repository contains the custom scripts used to identify poly(UG)-tailed RNAs in small RNA sequencing data.
If you use these scripts in your research, we kindly ask that you cite our paper:
Title: poly(UG)-tailed RNAs in genome protection and epigenetic inheritance
Author: Shukla A, Yan J, Pagano D, Dodson A, Fei Y, Gorham J, Seidman J, Wickens M, Kennedy S
Journal: Nature, 582, 283-288 (2020)
#1. Example for filter_barcodes.py:
python filter_barcodes.py -i input.fastq -o output.fastq
#2. Example for filter_22G_reads.py:
python filter_22G_reads.py -i input.fastq -o output.fastq
#3. Example for filter_protein_coding.sh:
sh filter_protein_coding.sh