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Contributor submission GelB2 #155

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80 changes: 80 additions & 0 deletions mite_data/data/fe330d38-e3e9-11f0-abdb-8218ec668ab3.json
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{
"accession": "MITE9999999",
"status": "pending",
"comment": "Enzyme identifier was found by manual search for precursor peptide in genome of Brevibacillus gelatini strain DSM 100115",
"changelog": [
{
"version": "1",
"date": "2025-12-28",
"contributors": [
"AAAAAAAAAAAAAAAAAAAAAAAA"
],
"reviewers": [
"BBBBBBBBBBBBBBBBBBBBBBBB"
],
"comment": "New entry."
}
],
"enzyme": {
"name": "GelB2",
"description": "Peptidase",
"references": [
"doi:10.1002/anie.202425134",
"doi:10.64898/2025.12.02.691826"
],
"auxiliaryEnzymes": [
{
"name": "GelA",
"description": "Precursor peptide",
"databaseIds": {
"uniprot": "UPI0026784BDC"
}
},
{
"name": "GelB1",
"description": "Stand-alone ribosomal recognition element",
"databaseIds": {
"uniprot": "UPI000F0895EA"
}
},
{
"name": "GelC",
"description": "Macrolactam synthetase",
"databaseIds": {
"uniprot": "UPI000F08A2CC"
}
}
],
"databaseIds": {
"uniprot": "UPI000F088A46"
}
},
"reactions": [
{
"tailoring": [
"Hydrolysis"
],
"description": "N-terminal cleavage of gelatinamin leader peptide, resulting in immature intermediate core peptide.",
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"reactions": [
{
"substrate": "CSCC[C@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CCSC)C(C)C)C(C)C)[C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CC(=O)O)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)NCC(=O)N[C@@H](CCCNC(=N)N)C(=O)NCC(=O)N[C@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)O)C(C)C)[C@@H](C)O)C(C)C",
"products": [
"CC(C)[C@H](NC(=O)CNC(=O)[C@H](CCCNC(=N)N)NC(=O)CNC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@H](CC(=O)O)NC(=O)CNC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)O"
],
"isIntermediate": true,
"description": "Precursor cleavage of the GelA precursor peptide, resulting in the intermediate core peptide before macrolactam formation."
}
],
"evidence": {
"evidenceCode": [
"Heterologous expression",
"Knock-out studies"
],
"references": [
"doi:10.64898/2025.12.02.691826"
]
}
}
]
}