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23 changes: 15 additions & 8 deletions README.md
Original file line number Diff line number Diff line change
Expand Up @@ -21,24 +21,31 @@ TACI_Python_version is a python-based 3D calcium imaging analysis pipeline used

---

## Installation and python testing
## Installation and Python Testing

1. Clone or download this repository.
2. Create a python virtrual environment.
2. Create a Python virtual environment.
3. Install dependencies:

```bash
python -m pip install --upgrade pip
```
```bash
python -m pip install -r requirements.txt
4. Test scripts usging demo data
```
4. Test scripts using demo data:
```bash
python .\CIAanalysis_120min.py -i path/demo_analysis --merge --cell_type DOWC
python .\CITbind_dynamic.py -i path/demo_cbind -n 2

python CIAanalysis_120min.py -i path/demo_analysis --merge --cell_type DOWC
```
```bash
python CITbind_dynamic.py -i path/demo_cbind -n 2
```

## Description
These python scripts allows batch processing and analysis of calcium imaging datasets collected from Drosophila larvae or other small model systems.
The pipline has three main stages:
1. Fluorescence extraction using TrackMate in ImageJ<br>
insert the user manual for the steps in Trackmate here!
1. Fluorescence Extraction (via TrackMate in ImageJ)<br>
(Insert user manual here for steps in ImageJ TrackMate output generation.)<br>
2. Primary Analysis (CIAnalysis_120min.py)<br>
This is the main script for the processing individual calcium imaging datasets.<br>
It automatially:
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