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This repository aims to host robust and consistent tools for MS based proteomics data analysis with Python

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pyMSpro

Proteomics Mass Spectrometry based Data Analysis

Welcome to the repository for my Master Thesis in Biomedical Data Science. This repository hosts the code, tools, and data analysis performed as part of my thesis work. The project focuses on the analysis of MS proteomic data from various studies.

Contents

  • Python Modules: Custom mdules developed to assist with data analysis.
  • Data Analysis Notebook: Jupyter Notebook with detailed data analysis of the datasets listed below.

Datasets

The following datasets were analyzed in this project:

Study Type Method*
Iwata, H., et al. (2016) Bulk DDA
Li, P., et al. (2022) Bulk DDA
Li, P., et al. (2021) Bulk DDA
Huffman, R. G., et al. (2022) Single Cell DIA

*Method: DDA (Data-Dependent Acquisition) or DIA (Data-Independent Acquisition)

Project Structure

The repository is structured as follows:

  • /src: Contains custom Python tools and modules developed for data analysis.
  • /results: Generated HTML files with the results of the network analysis.
  • /data: Contains the datasets used in the analysis.
  • /QC_*.R: Quality Control files in R.

Usage

To use the tools and run the analysis, follow these steps:

  1. Clone the repository:

    git clone https://github.com/powwowath/pyMSpro.git
    cd pyMSpro
  2. Install dependencies:

    pip install -r requirements.txt
  3. Run the analysis: Open the Notebook "00_workflow_META-ANALYSIS.ipynb" and follow the instructions to perform the analysis.

Contact

For any questions or further information, please contact me at [gerard.font@estudiants.urv.cat] or [ath@athzone.com].


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This repository aims to host robust and consistent tools for MS based proteomics data analysis with Python

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