This project used single cell RNA sequencing with SmartSeq2 to compare the transcriptional profile of tumors derived from type 1 alveolar epithelial cells with those derived from type 2 alveolar epithelial cells.
- SW (Sftpc-CreER-rtTA, mTmG) cells are from a mouse in which type 2 alveolar epithelial cells with wildtype Kras were isolated 18 days after tamoxifen was given.
- SK (Sftpc-CreER-rtTA, LSL-KrasG12D, mTmG) cells are from a mouse in which AT2 lineage traced cells with the Kras G12D mutation were isolated 18 days after tamoxifen was given.
- HW (Hopx-CreER, mTmG) cells are from a mouse in which alveolar type 1 (AT1) and alveolar type 2 (AT2) cells with wildtype Kras were isolated 10 months and 18 days after tamoxifen was given.
- HK (Hopx-CreER, LSL-KrasG12D, mTmG) cells are from a mouse in which alveolar type 1 (AT1) lineage traced cells with the KrasG12D mutation were isolated 10 months and 18 days after tamoxifen was given.
Data for this project are available at Synapse here.
fastq files for this dataset will be deposited with the BioProject accession number PRJNA975973.