Tutorials

• Tutorial 1: Analysis and output for single files using intensity thresholding

  Learn how to analyze single image stacks by applying intensity thresholds to select pixels of interest and export results in various formats. This tutorial is highly recommended for beginners.
  Watch Tutorial 1

• Tutorial 2: Masks and ROI management

  Learn how to manage specific regions by using predefined binary masks, manual ROI drawing tools, or imported ROI files.
  Watch Tutorial 2

• Tutorial 3: Batch analysis for multiple files

  Learn how to automate the processing of multiple image stacks and paired masks/ROIs within a single folder for high-throughput analysis.
  Watch Tutorial 3

• Tutorial 4: Normalizing ρ values with respect to a reference angle or boundary

  Learn how to calculate molecular orientations relative to a specific global reference angle or local structural boundaries.
  Watch Tutorial 4

• Tutorial 5: Interactive figure exploration, cropping, and colorbar customization

  Learn how to refine data visualization through interactive figure tools, and synchronized cropping across variables.
  Watch Tutorial 5